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(2016). https://pubmed.ncbi.nlm.nih.gov/27307040/ DOI: 10.1074/jbc.m116.734210","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ab87cda1-fd07-592d-a475-8925fde6d7f2","evidence_kind":"source_excerpt","locator":"Lines 350-361","start_line":350,"end_line":361,"excerpt":"### ve-transport-r221w-localization\nHuman TTP(R221W) remained punctate after alpha-tocopherol treatment in rat McARH7777 cells, unlike wild-type TTP.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An AVED-associated variant failed to change location when vitamin E was supplied.\norganism: Human proteins in Rattus norvegicus cells\ntissue_or_cell_type: Hepatoma cells\nexperimental_model: Human wild-type versus R221W TTP expression; Figure 2B\nlimitations: Mutation contrast is machinery impairment; this does not establish that supplementation cannot help AVED clinically.\nexposure: 35 µM serum-complexed alpha-tocopherol for 24 hours after medium depletion.\ncross_nutrient: false\n[chung2016] Vitamin E and Phosphoinositides Regulate the Intracellular Localization of the Hepatic α-Tocopherol Transfer Protein. 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