{"id":"17e8e2e3-4816-5979-8da5-12999fe17fa5","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-nka-isotope-opposed-flux","predicate":"mediates","statement":"Sodium-24 and potassium-42 experiments demonstrated ouabain-sensitive sodium efflux and potassium influx in human red cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2a4706d1-7acd-5a3f-93e4-b6906d73f82a","mechanism_event_label":"The pump moves the two nutrient ions in opposite directions.","subject":{"id":"27c3e7bd-c6e1-5344-8130-b231a9da2cfb","slug":"sodium-potassium-atpase","display_name":"Sodium-potassium ATPase complexes","entity_type_key":"protein_family"},"object":{"id":"10d562c8-194e-5be1-8dac-859ab2a9a587","slug":"sodium-pump-potassium-influx","display_name":"Sodium-pump-mediated potassium influx","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2a4706d1-7acd-5a3f-93e4-b6906d73f82a","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-nka-isotope-opposed-flux-event","event_type":"biochemical_relationship","label":"The pump moves the two nutrient ions in opposite directions.","description":"Sodium-24 and potassium-42 experiments demonstrated ouabain-sensitive sodium efflux and potassium influx in human red cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"outward tracer ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"inward tracer ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"energy substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"27c3e7bd-c6e1-5344-8130-b231a9da2cfb","slug":"sodium-potassium-atpase","display_name":"Sodium-potassium ATPase complexes","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"10d562c8-194e-5be1-8dac-859ab2a9a587","slug":"sodium-pump-potassium-influx","display_name":"Sodium-pump-mediated potassium influx","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium-dependent ATP chemistry is coupled to sodium and potassium handling by the pump; serum magnesium is not the enzyme-site concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human red cells and resealed ghosts; ATP/Mg loading, ouabain, sodium-24, potassium-42 and gamma-phosphorus-32 ATP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This experiment found unequal fluxes but did not itself measure an exact 3:2 ratio.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The pump moves the two nutrient ions in opposite directions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[garrahan-1967-nka] The stoicheiometry of the sodium pump (1967). https://doi.org/10.1113/jphysiol.1967.sp008297 DOI: 10.1113/jphysiol.1967.sp008297","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Erythrocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_direction","value_text":"Sodium outward; potassium inward.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b374e6c5-540b-540e-8682-86d268540815","evidence_kind":"source_excerpt","locator":"Lines 832-843","start_line":832,"end_line":843,"excerpt":"### mg-nka-isotope-opposed-flux\nSodium-24 and potassium-42 experiments demonstrated ouabain-sensitive sodium efflux and potassium influx in human red cells.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The pump moves the two nutrient ions in opposite directions.\norganism: Homo sapiens\ntissue_or_cell_type: Erythrocytes\nexperimental_model: Human red cells and resealed ghosts; ATP/Mg loading, ouabain, sodium-24, potassium-42 and gamma-phosphorus-32 ATP.\nlimitations: This experiment found unequal fluxes but did not itself measure an exact 3:2 ratio.\ncross_nutrient: Magnesium-dependent ATP chemistry is coupled to sodium and potassium handling by the pump; serum magnesium is not the enzyme-site concentration.\ntransport_direction: Sodium outward; potassium inward.\n[garrahan-1967-nka] The stoicheiometry of the sodium pump (1967). https://doi.org/10.1113/jphysiol.1967.sp008297 DOI: 10.1113/jphysiol.1967.sp008297","model_system":"Human red cells and resealed ghosts; ATP/Mg loading, ouabain, sodium-24, potassium-42 and gamma-phosphorus-32 ATP.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [garrahan-1967-nka] The stoicheiometry of the sodium pump (1967). https://doi.org/10.1113/jphysiol.1967.sp008297 DOI: 10.1113/jphysiol.1967.sp008297","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}