{"id":"1747e4c9-c62e-5af2-b350-6ee116474b5a","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:saccharopine-mitochondrial-toxicity","predicate":"damages","statement":"Excess saccharopine disrupts mitochondrial morphology and function in AASS-domain mutant models.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c1fbc2b4-cad0-5d03-bdee-f126414b775d","mechanism_event_label":"Accumulated saccharopine can harm mitochondria.","subject":{"id":"d70bb0d3-bb08-5b47-9ac0-5c7d8b56dd24","slug":"saccharopine","display_name":"Saccharopine","entity_type_key":"small_molecule"},"object":{"id":"d12d127a-a7d8-50f5-9edd-e47b5fbe81b7","slug":"mitochondrial-homeostasis","display_name":"Mitochondrial homeostasis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c1fbc2b4-cad0-5d03-bdee-f126414b775d","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:saccharopine-mitochondrial-toxicity-event","event_type":"biochemical_relationship","label":"Accumulated saccharopine can harm mitochondria.","description":"Excess saccharopine disrupts mitochondrial morphology and function in AASS-domain mutant models.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d70bb0d3-bb08-5b47-9ac0-5c7d8b56dd24","slug":"saccharopine","display_name":"Saccharopine","entity_type_key":"small_molecule"},"role":"accumulating_metabolite","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d12d127a-a7d8-50f5-9edd-e47b5fbe81b7","slug":"mitochondrial-homeostasis","display_name":"Mitochondrial homeostasis","entity_type_key":"cellular_process"},"role":"affected_process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"affected_machinery","value_text":"AASS","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"deficiency_not_equivalent","value_text":"Dietary lysine deficiency","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Genetic and suppression experiments in worms and mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Evidence is model-specific, not a toxicity threshold for normal human lysine intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Caenorhabditis elegans and Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Accumulated saccharopine can harm mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zhou2019] The lysine catabolite saccharopine impairs development by disrupting mitochondrial homeostasis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6363459/ DOI: 10.1083/jcb.201807204","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Worm hypodermis and mouse liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"03cf321d-20ea-5eb8-a9f0-74477032835f","evidence_kind":"source_excerpt","locator":"Lines 242-252","start_line":242,"end_line":252,"excerpt":"### saccharopine-mitochondrial-toxicity\nExcess saccharopine disrupts mitochondrial morphology and function in AASS-domain mutant models.\nPlain language: Accumulated saccharopine can harm mitochondria.\nCondition category: machinery_impairment\norganism: Caenorhabditis elegans and Mus musculus\ntissue_or_cell_type: Worm hypodermis and mouse liver\nexperimental_model: Genetic and suppression experiments in worms and mice\nlimitations: Evidence is model-specific, not a toxicity threshold for normal human lysine intake.\naffected_machinery: AASS\ndeficiency_not_equivalent: Dietary lysine deficiency\n[zhou2019] The lysine catabolite saccharopine impairs development by disrupting mitochondrial homeostasis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6363459/ DOI: 10.1083/jcb.201807204","model_system":"Genetic and suppression experiments in worms and mice","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [zhou2019] The lysine catabolite saccharopine impairs development by disrupting mitochondrial homeostasis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6363459/ DOI: 10.1083/jcb.201807204","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}