{"id":"17327d4d-6f4a-5836-9c53-87fb11ede83c","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:melatonin-stress","predicate":"context_dependent_observation","statement":"Melatonin accompanied changes in zinc balance, thymulin and IL-2 in stressed tumor-bearing mice; co-movement did not establish thymulin mediation of tumor outcomes.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2e51f4d0-bdea-5764-8bf8-79f7fa86fae3","mechanism_event_label":"Melatonin accompanied changes in zinc balance, thymulin and IL-2 in stressed tumor-bearing mice; co-movement did not establish thymulin mediation of tumor outcomes.","subject":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"object":{"id":"8cf03312-bba2-5625-b1ad-abc85d7bddc5","slug":"mouse-thymulin-bioactivity","display_name":"Mouse thymulin bioassay activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2e51f4d0-bdea-5764-8bf8-79f7fa86fae3","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:melatonin-stress-event","event_type":"experimental_observation","label":"Melatonin accompanied changes in zinc balance, thymulin and IL-2 in stressed tumor-bearing mice; co-movement did not establish thymulin mediation of tumor outcomes.","description":"| Melatonin and stress | In a stressed, tumor-bearing mouse model, melatonin treatment accompanied changes in zinc balance, thymulin and IL-2. Co-movement does not prove a direct melatonin → thymulin receptor pathway or that thymulin mediated the tumor outcome. [1999 study](https://pubmed.ncbi.nlm.nih.gov/10411280/) |","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0f0bc1f1-d18d-5652-bc1d-98f61f7ab65c","slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Melatonin during stress","sequence_order":0,"notes":""},{"entity":{"id":"8cf03312-bba2-5625-b1ad-abc85d7bddc5","slug":"mouse-thymulin-bioactivity","display_name":"Mouse thymulin bioassay activity","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"mixed","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"separately measured zinc balance","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"peptide activity","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Melatonin during stress\", \"comparator\": \"Study control\", \"endpoint\": \"Melatonin accompanied changes in zinc balance, thymulin and IL-2 in stressed tumor-bearing mice; co-movement did not establish thymulin mediation of tumor outcomes.\", \"effect_direction\": \"mixed\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Stressed tumor-bearing mouse model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Melatonin accompanied changes in zinc balance, thymulin and IL-2 in stressed tumor-bearing mice; co-movement did not establish thymulin mediation of tumor outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Melatonin administration in tumor-bearing mice (intact and pinealectomized) in relation to stress, zinc, thymulin and IL-2. | 1999 | DOI 10.1016/s0192-0561(98)00067-8 | PMID 10411280 | https://pubmed.ncbi.nlm.nih.gov/10411280/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 96-96; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c17b287c-7173-5376-80b0-ae1a3f921587","evidence_kind":"source_excerpt","locator":"Lines 96-96","start_line":96,"end_line":96,"excerpt":"| Melatonin and stress | In a stressed, tumor-bearing mouse model, melatonin treatment accompanied changes in zinc balance, thymulin and IL-2. Co-movement does not prove a direct melatonin → thymulin receptor pathway or that thymulin mediated the tumor outcome. [1999 study](https://pubmed.ncbi.nlm.nih.gov/10411280/) |","model_system":"Stressed tumor-bearing mouse model.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e1871d49-9c6b-547b-a1bb-6e2b9270ca89","stable_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"6e8ed0d7a5e23fc822f2ab418e0c4fb2411c01fcaa330039e8d7d93218cacc88","revision_id":"8c0e3449-9bbd-57c0-bb5e-de83f044f2ac","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}