{"id":"16f3d990-9c37-5ac1-9420-7e5e98943c19","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-gtpbp3-respiration","predicate":"knockout_impairs","statement":"GTPBP3-knockout human cells exhibited impaired mitochondrial respiration alongside loss of taurine tRNA modification.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f1b0d823-6361-586e-a433-8e72d9ea528c","mechanism_event_label":"The RNA-processing defect reached the cell respiratory machinery.","subject":{"id":"cd2a6b5d-a6cf-5829-b0e4-b52a0f92d153","slug":"gtpbp3","display_name":"Human mitochondrial GTP-binding protein 3 / GTPBP3","entity_type_key":"protein"},"object":{"id":"ae4917ca-ced2-5ebe-a419-0f99953e4d56","slug":"human-gtpbp3-respiration","display_name":"Mitochondrial respiration in human GTPBP3-deficient cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f1b0d823-6361-586e-a433-8e72d9ea528c","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-gtpbp3-respiration-event","event_type":"observed_relationship","label":"The RNA-processing defect reached the cell respiratory machinery.","description":"GTPBP3-knockout human cells exhibited impaired mitochondrial respiration alongside loss of taurine tRNA modification.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cd2a6b5d-a6cf-5829-b0e4-b52a0f92d153","slug":"gtpbp3","display_name":"Human mitochondrial GTP-binding protein 3 / GTPBP3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ae4917ca-ced2-5ebe-a419-0f99953e4d56","slug":"human-gtpbp3-respiration","display_name":"Mitochondrial respiration in human GTPBP3-deficient cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell knockout; respiratory measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Same perturbation as the translation claim, not independent clinical evidence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The RNA-processing defect reached the cell respiratory machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29390138/ · DOI 10.1093/nar/gky068","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"7a505d26-c4d8-5548-9f5c-d62b85e89512","evidence_kind":"source_excerpt","locator":"Lines 257-263","start_line":257,"end_line":263,"excerpt":"## taurine-gtpbp3-respiration\nThe RNA-processing defect reached the cell respiratory machinery.\nGTPBP3-knockout human cells exhibited impaired mitochondrial respiration alongside loss of taurine tRNA modification.\nModel: Human cell knockout; respiratory measurements.\nLimitations: Same perturbation as the translation claim, not independent clinical evidence.\nEvidence access: Primary abstract\nMetabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29390138/ · DOI 10.1093/nar/gky068","model_system":"Human cell knockout; respiratory measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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