{"id":"16d9e93d-b071-53e0-ada2-7949494ade0d","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-binding","predicate":"decreases_in_recorded_comparison","statement":"Apigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"28c2d3d4-a183-5703-81a8-fc223b6cd61b","mechanism_event_label":"Apigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"5be96089-ad2d-5651-b94b-ff3a476da882","slug":"benzodiazepine-site","display_name":"The modulatory benzodiazepine-binding site of the GABA-A receptor","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"28c2d3d4-a183-5703-81a8-fc223b6cd61b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-binding-event","event_type":"biochemical_relationship","label":"Apigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study.","description":"### api-gaba-binding\n\nApigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study.\nNo muscimol-binding, muscarinic or alpha-1-adrenergic binding effects were reported in the tested assays. No proven alpha-1-sparing clinical receptor profile follows.\nModel and exposure: Central benzodiazepine-site binding assay; primary abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin, a component of Matricaria recutita flowers, is a central benzodiazepine receptors-ligand with anxiolytic effects. | 1995 | https://pubmed.ncbi.nlm.nih.gov/7617761/ | https://doi.org/10.1055/s-2006-958058 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin concentration series","sequence_order":0,"notes":""},{"entity":{"id":"5be96089-ad2d-5651-b94b-ff3a476da882","slug":"benzodiazepine-site","display_name":"The modulatory benzodiazepine-binding site of the GABA-A receptor","entity_type_key":"protein_state"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"5e42971b-427f-5b32-968b-870b347135ab","slug":"gaba-a-receptors","display_name":"GABA-A receptor anion channels","entity_type_key":"protein_family"},"role":"receptor containing the binding site","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin concentration series\", \"comparator\": \"Binding assay without competitor\", \"endpoint\": \"Apigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Central benzodiazepine-site binding assay; primary abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Apigenin, a component of Matricaria recutita flowers, is a central benzodiazepine receptors-ligand with anxiolytic effects. | https://pubmed.ncbi.nlm.nih.gov/7617761/ | https://doi.org/10.1055/s-2006-958058","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-gaba-binding; No muscimol-binding, muscarinic or alpha-1-adrenergic binding effects were reported in the tested assays. No proven alpha-1-sparing clinical receptor profile follows.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"44250281-77cd-58e5-bc4d-62828a1984c8","evidence_kind":"source_excerpt","locator":"Lines 780-786","start_line":780,"end_line":786,"excerpt":"### api-gaba-binding\n\nApigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study.\nNo muscimol-binding, muscarinic or alpha-1-adrenergic binding effects were reported in the tested assays. No proven alpha-1-sparing clinical receptor profile follows.\nModel and exposure: Central benzodiazepine-site binding assay; primary abstract.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin, a component of Matricaria recutita flowers, is a central benzodiazepine receptors-ligand with anxiolytic effects. | 1995 | https://pubmed.ncbi.nlm.nih.gov/7617761/ | https://doi.org/10.1055/s-2006-958058 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Central benzodiazepine-site binding assay; primary abstract.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[{"id":"57bd48b8-b81a-5b87-b6a4-49f65263530c","relation_type":"limits_scope_of","notes":"Binding-site affinity does not determine the sign of functional modulation.","source_claim_id":"afad15a9-b5aa-58bc-8921-01b89ab01b9b","source_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-current","source_statement":"Apigenin decreased GABA-activated chloride currents in cultured cerebellar granule cells; flumazenil antagonized that effect.","target_claim_id":"16d9e93d-b071-53e0-ada2-7949494ade0d","target_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gaba-binding","target_statement":"Apigenin displaced flunitrazepam binding with reported Ki 4 micromolar in the 1995 receptor-binding study."}],"conflicts":[],"corrections":[],"research":null}