{"id":"1612e432-74ea-5a52-8373-dfc8874be35b","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:raw-nfkb-null","predicate":"no_detected_change_in_recorded_experiment","statement":"Thymulin did not reduce the reported NF-kappa-B activation readout in LPS-stimulated RAW264.7 macrophages; this is not evidence for unconditional NF-kappa-B inhibition.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"9adbaa0a-49b2-5868-ba92-db5c5a9495ef","mechanism_event_label":"Thymulin did not reduce the reported NF-kappa-B activation readout in LPS-stimulated RAW264.7 macrophages; this is not evidence for unconditional NF-kappa-B inhibition.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"51ccf7f9-d3b9-5ef7-83ca-78a14399ea34","slug":"mouse-nfkb-pathway","display_name":"Mouse NF-kappa-B signaling readouts","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9adbaa0a-49b2-5868-ba92-db5c5a9495ef","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:raw-nfkb-null-event","event_type":"experimental_observation","label":"Thymulin did not reduce the reported NF-kappa-B activation readout in LPS-stimulated RAW264.7 macrophages; this is not evidence for unconditional NF-kappa-B inhibition.","description":"| RAW264.7 macrophages, unstimulated versus LPS-stimulated | In resting cells, thymulin increased proinflammatory cytokine secretion. In LPS-stimulated cells, it reduced SAPK/JNK activity but not NF-κB activity; peptide alone did not change the measured cytokine secretion. HSP72 increased. | Preserve the resting/stimulated contrast. Thymopentin was also studied and requires separate attribution. [2011 experiment](https://pubmed.ncbi.nlm.nih.gov/22148922/) |","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Thymulin plus LPS","sequence_order":0,"notes":""},{"entity":{"id":"51ccf7f9-d3b9-5ef7-83ca-78a14399ea34","slug":"mouse-nfkb-pathway","display_name":"Mouse NF-kappa-B signaling readouts","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"added peptide","comparator":"LPS alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Thymulin plus LPS\", \"comparator\": \"LPS alone\", \"endpoint\": \"Thymulin did not reduce the reported NF-kappa-B activation readout in LPS-stimulated RAW264.7 macrophages; this is not evidence for unconditional NF-kappa-B inhibition.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"thymulin\", \"state\": \"added peptide\"}, {\"entity_slug\": null, \"state\": \"LPS stimulation\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mouse RAW264.7 macrophages; LPS stimulation; endpoint retained at reported pathway level.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. 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