{"id":"1601365b-a70a-5902-817f-8d7a52fddde3","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-n3-emotional-behaviour","predicate":"impairs","statement":"The dietary-induced reduction of CB1 receptor function in mood-controlling structures was associated with impaired emotional behaviour, identifying a plausible synaptic substrate for the behavioural alterations reported with n-3 deficiency in western diets.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c9fbcf11-52b9-56a4-8689-9f00e9a47d20","mechanism_event_label":"The animals behaved differently, and the synapse gives a reason why.","subject":{"id":"a32a52f5-b197-5d27-98a5-925024fdd968","slug":"n3-pufa-deficiency","display_name":"Lifelong dietary insufficiency of n-3 polyunsaturated fatty acids","entity_type_key":"cellular_process"},"object":{"id":"0b12b6d8-1735-57b9-8394-2283bf7d374a","slug":"emotional-behaviour","display_name":"Emotional behaviour in the tested mouse assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c9fbcf11-52b9-56a4-8689-9f00e9a47d20","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-n3-emotional-behaviour-event","event_type":"biochemical_relationship","label":"The animals behaved differently, and the synapse gives a reason why.","description":"The dietary-induced reduction of CB1 receptor function in mood-controlling structures was associated with impaired emotional behaviour, identifying a plausible synaptic substrate for the behavioural alterations reported with n-3 deficiency in western diets.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a32a52f5-b197-5d27-98a5-925024fdd968","slug":"n3-pufa-deficiency","display_name":"Lifelong dietary insufficiency of n-3 polyunsaturated fatty acids","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0b12b6d8-1735-57b9-8394-2283bf7d374a","slug":"emotional-behaviour","display_name":"Emotional behaviour in the tested mouse assays","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/thc-research/21278728.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d19499dcf42cdfc31c0fc46420a1bc84babcbfcd366cf1cb37f3fed8f5d38281\", \"start_char\": 0, \"end_char\": 953, \"text_sha256\": \"d19499dcf42cdfc31c0fc46420a1bc84babcbfcd366cf1cb37f3fed8f5d38281\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Lifelong n-3 deficient diet in mice with synaptic recordings and receptor coupling assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lifelong dietary insufficiency of n-3 polyunsaturated fatty acids","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A dietary manipulation with a molecular readout, receptor-effector uncoupling, rather than only a behavioural one. It is a lifelong deficiency, not a short-term change.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"THC research collection; topical membership is not evidence of a direct clinical effect, and THC is recorded separately from the endocannabinoids it imitates.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tetrahydrocannabinol","display_name":"Delta-9-tetrahydrocannabinol / THC","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The animals behaved differently, and the synapse gives a reason why.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[thc-p21278728] Nutritional omega-3 deficiency abolishes endocannabinoid-mediated neuronal functions. (2011). https://pubmed.ncbi.nlm.nih.gov/21278728/ DOI: 10.1038/nn.2736","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Prelimbic prefrontal cortex and accumbens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"6d7d57e1-5806-5fb2-8d01-ddd0b2d7f32b","evidence_kind":"source_excerpt","locator":"Lines 647-658","start_line":647,"end_line":658,"excerpt":"### thc-n3-emotional-behaviour\nThe dietary-induced reduction of CB1 receptor function in mood-controlling structures was associated with impaired emotional behaviour, identifying a plausible synaptic substrate for the behavioural alterations reported with n-3 deficiency in western diets.\nCondition category: nutrient_deficiency\nnutrient_topic: THC research collection; topical membership is not evidence of a direct clinical effect, and THC is recorded separately from the endocannabinoids it imitates.\nplain_language: The animals behaved differently, and the synapse gives a reason why.\norganism: Mouse\ntissue_or_cell_type: Prelimbic prefrontal cortex and accumbens\nexperimental_model: Lifelong n-3 deficient diet in mice with synaptic recordings and receptor coupling assays\nlimitations: A dietary manipulation with a molecular readout, receptor-effector uncoupling, rather than only a behavioural one. It is a lifelong deficiency, not a short-term change.\nexposure: Lifelong dietary insufficiency of n-3 polyunsaturated fatty acids\nevidence_span: {\"source_cache\": \"artifacts/thc-research/21278728.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d19499dcf42cdfc31c0fc46420a1bc84babcbfcd366cf1cb37f3fed8f5d38281\", \"start_char\": 0, \"end_char\": 953, \"text_sha256\": \"d19499dcf42cdfc31c0fc46420a1bc84babcbfcd366cf1cb37f3fed8f5d38281\"}\n[thc-p21278728] Nutritional omega-3 deficiency abolishes endocannabinoid-mediated neuronal functions. (2011). https://pubmed.ncbi.nlm.nih.gov/21278728/ DOI: 10.1038/nn.2736","model_system":"Lifelong n-3 deficient diet in mice with synaptic recordings and receptor coupling assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [thc-p21278728] Nutritional omega-3 deficiency abolishes endocannabinoid-mediated neuronal functions. (2011). https://pubmed.ncbi.nlm.nih.gov/21278728/ DOI: 10.1038/nn.2736","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b4367320-9724-507a-bead-6932fa0a78cb","stable_key":"import-05a3cf47-e660-5ab3-8c32-5eba9847cf0a","title":"THC: the cannabinoid receptors, the endocannabinoid system it occupies, what the drug does, and the dietary fat it is built from (2026-09-21)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"60abc81737d18b7a50b01ca487e38291774ee15d9777651aa2d3eac8670ac3b2","revision_id":"2cba3552-af89-580d-b20a-8cfb1b14f9e7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}