{"id":"15dc2dd3-b068-5eb0-9dd7-3e0f70770256","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-albumin","predicate":"binds","statement":"Human albumin bound NO2-CLA noncovalently and formed covalent adducts at Cys34.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f1a0eea3-1554-5eb1-8311-685bec5cab56","mechanism_event_label":"A circulating carrier can bind the modified lipid in two ways.","subject":{"id":"806c5846-1ca4-5854-b68d-699af6b447fb","slug":"nitro-conjugated-linoleic-acid","display_name":"Nitro-conjugated linoleic acid / NO2-CLA family","entity_type_key":"chemical_species"},"object":{"id":"d74adc7c-c253-5604-99bf-73d5ed0c0701","slug":"human-serum-albumin","display_name":"Human serum albumin","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f1a0eea3-1554-5eb1-8311-685bec5cab56","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-albumin-event","event_type":"observed_relationship","label":"A circulating carrier can bind the modified lipid in two ways.","description":"Human albumin bound NO2-CLA noncovalently and formed covalent adducts at Cys34.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"806c5846-1ca4-5854-b68d-699af6b447fb","slug":"nitro-conjugated-linoleic-acid","display_name":"Nitro-conjugated linoleic acid / NO2-CLA family","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d74adc7c-c253-5604-99bf-73d5ed0c0701","slug":"human-serum-albumin","display_name":"Human serum albumin","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified protein and chemical assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Distribution was a mechanistic implication, not a measured clinical outcome.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A circulating carrier can bind the modified lipid in two ways.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Chemical Basis of Thiol Addition to Nitro-conjugated Linoleic Acid, a Protective Cell-signaling Lipid. · 2017 · https://pubmed.ncbi.nlm.nih.gov/27923813/ · DOI 10.1074/jbc.M116.756288","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"205e467a-6fd7-5ca5-9a84-554d01f5f66b","evidence_kind":"source_excerpt","locator":"Lines 350-356","start_line":350,"end_line":356,"excerpt":"## cla-albumin\nA circulating carrier can bind the modified lipid in two ways.\nHuman albumin bound NO2-CLA noncovalently and formed covalent adducts at Cys34.\nModel: Purified protein and chemical assays.\nLimitations: Distribution was a mechanistic implication, not a measured clinical outcome.\nEvidence access: Primary abstract\nThe Chemical Basis of Thiol Addition to Nitro-conjugated Linoleic Acid, a Protective Cell-signaling Lipid. · 2017 · https://pubmed.ncbi.nlm.nih.gov/27923813/ · DOI 10.1074/jbc.M116.756288","model_system":"Purified protein and chemical assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}