{"id":"15bc74c6-3cfa-5cab-87ca-433f59247f89","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-abca4-pe-flip","predicate":"transports","statement":"Reconstituted ABCA4 also transported phosphatidylethanolamine in the import orientation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8dddabf6-ba1e-547f-ad7b-71e103250788","mechanism_event_label":"The transporter can move PE as well as its retinal adduct.","subject":{"id":"e45ac8b4-0e4b-560d-b34f-6358fbae4ee3","slug":"abca4","display_name":"ABCA4 retinoid transporter","entity_type_key":"protein"},"object":{"id":"be4a52f4-f31c-59b7-b0da-4178fbb069c8","slug":"phosphatidylethanolamine","display_name":"Phosphatidylethanolamine","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"8dddabf6-ba1e-547f-ad7b-71e103250788","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:a-vision-abca4-pe-flip-event","event_type":"biochemical_relationship","label":"The transporter can move PE as well as its retinal adduct.","description":"Reconstituted ABCA4 also transported phosphatidylethanolamine in the import orientation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e45ac8b4-0e4b-560d-b34f-6358fbae4ee3","slug":"abca4","display_name":"ABCA4 retinoid transporter","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"be4a52f4-f31c-59b7-b0da-4178fbb069c8","slug":"phosphatidylethanolamine","display_name":"Phosphatidylethanolamine","entity_type_key":"lipid"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Fluorescent lipid transport","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Lipid transport alone does not prove the complete visual-cycle flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Purified/reconstituted transporter","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The transporter can move PE as well as its retinal adduct.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[quazi-2012] ABCA4 is an N-retinylidene-phosphatidylethanolamine and phosphatidylethanolamine importer (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3871175/ DOI: 10.1038/ncomms1927","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Model membranes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_direction","value_text":"lumen-equivalent leaflet to cytoplasmic-equivalent leaflet","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Recorded as transport in the import orientation in the reconstituted system.","entity":null},{"dimension":"transport_pool","value_text":"the cytoplasmic leaflet of the disc membrane","comparator":null,"unit":null,"notes":"Recorded as transport in the import orientation in the reconstituted system.","entity":null}],"evidence":[{"id":"13856de1-963a-5b9b-85a0-e393940d4d13","evidence_kind":"source_excerpt","locator":"Lines 813-823","start_line":813,"end_line":823,"excerpt":"### a-vision-abca4-pe-flip\nReconstituted ABCA4 also transported phosphatidylethanolamine in the import orientation.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The transporter can move PE as well as its retinal adduct.\norganism: Purified/reconstituted transporter\ntissue_or_cell_type: Model membranes\nexperimental_model: Fluorescent lipid transport\nlimitations: Lipid transport alone does not prove the complete visual-cycle flux.\ntransport_direction: lumen-equivalent leaflet to cytoplasmic-equivalent leaflet\n[quazi-2012] ABCA4 is an N-retinylidene-phosphatidylethanolamine and phosphatidylethanolamine importer (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3871175/ DOI: 10.1038/ncomms1927","model_system":"Fluorescent lipid transport","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [quazi-2012] ABCA4 is an N-retinylidene-phosphatidylethanolamine and phosphatidylethanolamine importer (2012). https://pmc.ncbi.nlm.nih.gov/articles/PMC3871175/ DOI: 10.1038/ncomms1927","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}