{"id":"1547091d-dc93-5dc9-912e-78f4dd233377","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cpt2-return","predicate":"reversibly_transfers_acyl_groups","statement":"Expressed human CPT2 formed medium- and long-chain acylcarnitines from acyl-CoAs; its physiological matrix-side reaction reconverts incoming acylcarnitines to acyl-CoAs plus free carnitine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"905cd13a-d2a2-5922-9c07-5a3edce5bf79","mechanism_event_label":"The inner enzyme unloads the fatty-acid group and recycles carnitine.","subject":{"id":"ba546852-649c-5632-a58d-702bc82bb6f5","slug":"human-cpt2","display_name":"Human carnitine palmitoyltransferase 2 / CPT2","entity_type_key":"protein"},"object":{"id":"f1c881ce-8094-5602-bb97-074377340185","slug":"palmitoylcarnitine","display_name":"Palmitoyl-L-carnitine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"905cd13a-d2a2-5922-9c07-5a3edce5bf79","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cpt2-return-event","event_type":"observed_relationship","label":"The inner enzyme unloads the fatty-acid group and recycles carnitine.","description":"Expressed human CPT2 formed medium- and long-chain acylcarnitines from acyl-CoAs; its physiological matrix-side reaction reconverts incoming acylcarnitines to acyl-CoAs plus free carnitine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ba546852-649c-5632-a58d-702bc82bb6f5","slug":"human-cpt2","display_name":"Human carnitine palmitoyltransferase 2 / CPT2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f1c881ce-8094-5602-bb97-074377340185","slug":"palmitoylcarnitine","display_name":"Palmitoyl-L-carnitine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a6244ae7-59a4-5123-abd2-e5863be007b3","slug":"palmitoyl-coa","display_name":"Palmitoyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and reviewed UniProt catalytic-reaction record","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CPT2 in yeast homogenates; physiological direction from reviewed UniProt P23786/Rhea 12663.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The experiment directly measured the reverse reaction; direction in a cell depends on compartment and substrates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The inner enzyme unloads the fatty-acid group and recycles carnitine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnitine palmitoyltransferase 2: New insights on the substrate specificity and implications for acylcarnitine profiling. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20538056/ · DOI 10.1016/j.bbadis.2010.06.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"40d018dd-a1a7-5911-8c02-b43ef15f3958","evidence_kind":"source_excerpt","locator":"Lines 106-112","start_line":106,"end_line":112,"excerpt":"## l-carnitine-cpt2-return\nThe inner enzyme unloads the fatty-acid group and recycles carnitine.\nExpressed human CPT2 formed medium- and long-chain acylcarnitines from acyl-CoAs; its physiological matrix-side reaction reconverts incoming acylcarnitines to acyl-CoAs plus free carnitine.\nModel: Human CPT2 in yeast homogenates; physiological direction from reviewed UniProt P23786/Rhea 12663.\nLimitations: The experiment directly measured the reverse reaction; direction in a cell depends on compartment and substrates.\nEvidence access: Primary abstract and reviewed UniProt catalytic-reaction record\nCarnitine palmitoyltransferase 2: New insights on the substrate specificity and implications for acylcarnitine profiling. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20538056/ · DOI 10.1016/j.bbadis.2010.06.002","model_system":"Human CPT2 in yeast homogenates; physiological direction from reviewed UniProt P23786/Rhea 12663.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"6fcd86d9d02babb48b2ce658d506afe84580ad4b242f93f11f742d934fa5b361","revision_id":"398e3b52-45bc-52e3-891e-656c19ca674f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}