{"id":"153a3b3a-f8f1-50a6-808a-288b1d02cb72","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-pendrin-bicarbonate","predicate":"loss_of_function_impairs","statement":"Pendrin-null collecting ducts had reduced apical chloride/bicarbonate exchange and impaired bicarbonate secretion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a39a6fb1-d58c-5bba-a054-728677d6a167","mechanism_event_label":"The kidney uses chloride exchange to help dispose of bicarbonate.","subject":{"id":"00604217-ddba-5865-bd19-d2f4b013de6b","slug":"mouse-slc26a4","display_name":"Mouse pendrin / Slc26a4","entity_type_key":"protein"},"object":{"id":"eff6d1ae-bc8f-505f-9735-33454f7a850e","slug":"renal-bicarbonate-secretion","display_name":"Renal bicarbonate secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a39a6fb1-d58c-5bba-a054-728677d6a167","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-pendrin-bicarbonate-event","event_type":"biochemical_relationship","label":"The kidney uses chloride exchange to help dispose of bicarbonate.","description":"Pendrin-null collecting ducts had reduced apical chloride/bicarbonate exchange and impaired bicarbonate secretion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"exchange_partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"edf44f6b-7b23-5b97-a86b-c940e5812b06","slug":"bicarbonate-ion","display_name":"Bicarbonate ion","entity_type_key":"ion"},"role":"secreted_anion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"00604217-ddba-5865-bd19-d2f4b013de6b","slug":"mouse-slc26a4","display_name":"Mouse pendrin / Slc26a4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eff6d1ae-bc8f-505f-9735-33454f7a850e","slug":"renal-bicarbonate-secretion","display_name":"Renal bicarbonate secretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/20375274.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8ddda637993b80b24351aefe0451312d849175bd3ec2f3af23b72b50f7439953\", \"start_char\": 0, \"end_char\": 1797, \"text_sha256\": \"8ddda637993b80b24351aefe0451312d849175bd3ec2f3af23b72b50f7439953\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pendrin knockout and isolated collecting-duct perfusion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Slc26a4 deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Residual exchange remained; baseline urine chloride excretion was unchanged.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chloride research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chloride","display_name":"Chloride","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The kidney uses chloride exchange to help dispose of bicarbonate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chloride-p20375274] Deletion of the anion exchanger Slc26a4 (pendrin) decreases apical Cl(-)/HCO3(-) exchanger activity and impairs bicarbonate secretion in kidney collecting duct. (2010). https://pubmed.ncbi.nlm.nih.gov/20375274/ DOI: 10.1152/ajpcell.00033.2010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Non-acid-secreting intercalated cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"e833f098-b180-567d-8f99-81a662154f9c","evidence_kind":"source_excerpt","locator":"Lines 510-521","start_line":510,"end_line":521,"excerpt":"### chloride-pendrin-bicarbonate\nPendrin-null collecting ducts had reduced apical chloride/bicarbonate exchange and impaired bicarbonate secretion.\nCondition category: machinery_impairment\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The kidney uses chloride exchange to help dispose of bicarbonate.\norganism: Mouse\ntissue_or_cell_type: Non-acid-secreting intercalated cells\nexperimental_model: Pendrin knockout and isolated collecting-duct perfusion\nlimitations: Residual exchange remained; baseline urine chloride excretion was unchanged.\nexposure: Slc26a4 deletion\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/20375274.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"8ddda637993b80b24351aefe0451312d849175bd3ec2f3af23b72b50f7439953\", \"start_char\": 0, \"end_char\": 1797, \"text_sha256\": \"8ddda637993b80b24351aefe0451312d849175bd3ec2f3af23b72b50f7439953\"}\n[chloride-p20375274] Deletion of the anion exchanger Slc26a4 (pendrin) decreases apical Cl(-)/HCO3(-) exchanger activity and impairs bicarbonate secretion in kidney collecting duct. (2010). https://pubmed.ncbi.nlm.nih.gov/20375274/ DOI: 10.1152/ajpcell.00033.2010","model_system":"Pendrin knockout and isolated collecting-duct perfusion","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chloride-p20375274] Deletion of the anion exchanger Slc26a4 (pendrin) decreases apical Cl(-)/HCO3(-) exchanger activity and impairs bicarbonate secretion in kidney collecting duct. 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