{"id":"1521e192-2eb1-5993-8525-1516b124e842","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:gallic-walnut","predicate":"converted_to_in_recorded_reaction","statement":"Walnut shikimate dehydrogenase supports the studied oxidative route toward gallic acid.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"198ca29d-a4a8-5302-b46c-b4094616a3bc","mechanism_event_label":"Walnut shikimate dehydrogenase supports the studied oxidative route toward gallic acid.","subject":{"id":"320d1df4-8da0-5e1a-bb17-5140e264bf06","slug":"3-dehydroshikimate","display_name":"3-Dehydroshikimate / DHS","entity_type_key":"small_molecule"},"object":{"id":"3f57fdfc-6135-5103-acae-ce55206fca16","slug":"gallic-acid","display_name":"Gallic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"198ca29d-a4a8-5302-b46c-b4094616a3bc","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:gallic-walnut-event","event_type":"biochemical_reaction","label":"Walnut shikimate dehydrogenase supports the studied oxidative route toward gallic acid.","description":"**A plant oxidative branch is not a human metabolic pathway.** Walnut shikimate-dehydrogenase work and E. coli AroE assays support oxidation of DHS toward gallic acid with NADP+ in the studied enzyme systems; expression of walnut JrSDH in tobacco increased gallic-acid accumulation. This branch shows why substrate and redox context matter for enzyme function. It is not evidence that oral shikimic acid is converted into gallic acid by a human shikimate pathway. [Mechanism of gallic acid biosynthesis in bacteria (Escherichia coli) and walnut (Juglans regia).](https://pubmed.ncbi.nlm.nih.gov/21279669/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"320d1df4-8da0-5e1a-bb17-5140e264bf06","slug":"3-dehydroshikimate","display_name":"3-Dehydroshikimate / DHS","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3f57fdfc-6135-5103-acae-ce55206fca16","slug":"gallic-acid","display_name":"Gallic acid","entity_type_key":"small_molecule"},"role":"output","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"40b311bf-15e6-58b4-8ac6-f0687cd6e22d","slug":"walnut-shikimate-dehydrogenase","display_name":"Juglans regia shikimate dehydrogenase / JrSDH","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"output","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Walnut enzyme assays and transgenic tobacco context; not a human pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Walnut shikimate dehydrogenase supports the studied oxidative route toward gallic acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mechanism of gallic acid biosynthesis in bacteria (Escherichia coli) and walnut (Juglans regia). | 2011 | DOI 10.1007/s11103-011-9739-3 | PMID 21279669 | https://pubmed.ncbi.nlm.nih.gov/21279669/ | https://doi.org/10.1007/s11103-011-9739-3 | https://pmc.ncbi.nlm.nih.gov/articles/PMC3057006/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 87-87; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d3756ddb-7aa1-59ac-bb65-56e2f683674d","evidence_kind":"source_excerpt","locator":"Lines 87-87","start_line":87,"end_line":87,"excerpt":"**A plant oxidative branch is not a human metabolic pathway.** Walnut shikimate-dehydrogenase work and E. coli AroE assays support oxidation of DHS toward gallic acid with NADP+ in the studied enzyme systems; expression of walnut JrSDH in tobacco increased gallic-acid accumulation. This branch shows why substrate and redox context matter for enzyme function. It is not evidence that oral shikimic acid is converted into gallic acid by a human shikimate pathway. [Mechanism of gallic acid biosynthesis in bacteria (Escherichia coli) and walnut (Juglans regia).](https://pubmed.ncbi.nlm.nih.gov/21279669/)","model_system":"Walnut enzyme assays and transgenic tobacco context; not a human pathway.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}