{"id":"151130db-95a5-5799-af2d-34dfca28a982","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-neuroplastin-pmca1-support","predicate":"supports","statement":"Neuroplastin binds the transmembrane region of human PMCA1 and supports pump ATPase activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"167c0fcc-9333-59a7-ad8a-1632bd4f6a50","mechanism_event_label":"Neuroplastin helps PMCA1 function.","subject":{"id":"c4fa0bb7-fb71-5a56-8e5f-61a7dadc682c","slug":"nptn","display_name":"Neuroplastin / NPTN","entity_type_key":"protein"},"object":{"id":"dd68a62e-78df-5944-a783-48b9818aeed1","slug":"atp2b1","display_name":"Plasma membrane calcium ATPase 1 / ATP2B1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"167c0fcc-9333-59a7-ad8a-1632bd4f6a50","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-neuroplastin-pmca1-support-event","event_type":"regulation","label":"Neuroplastin helps PMCA1 function.","description":"Neuroplastin binds the transmembrane region of human PMCA1 and supports pump ATPase activity.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"c4fa0bb7-fb71-5a56-8e5f-61a7dadc682c","slug":"nptn","display_name":"Neuroplastin / NPTN","entity_type_key":"protein"},"role":"auxiliary_subunit","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dd68a62e-78df-5944-a783-48b9818aeed1","slug":"atp2b1","display_name":"Plasma membrane calcium ATPase 1 / ATP2B1","entity_type_key":"protein"},"role":"regulated_pump","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f0b6bcb6-5d41-5d9d-af9f-6af1a94d5c5a","slug":"plasma-membrane","display_name":"Plasma membrane","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Plasma membrane protein complex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human PMCA1-neuroplastin complex; cryo-EM and ATPase assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified PMCA1 complex; subunit availability and other PMCA isoforms require separate evidence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Neuroplastin helps PMCA1 function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-gong2018] Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin (2018). https://pubmed.ncbi.nlm.nih.gov/30190470/ DOI: 10.1038/s41467-018-06075-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"regulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant proteins","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dfb43e88-5b44-5e8e-a52a-945695e4f290","evidence_kind":"source_excerpt","locator":"Lines 599-610","start_line":599,"end_line":610,"excerpt":"### ca-neuroplastin-pmca1-support\nNeuroplastin binds the transmembrane region of human PMCA1 and supports pump ATPase activity.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Neuroplastin helps PMCA1 function.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant proteins\nexperimental_model: Purified human PMCA1-neuroplastin complex; cryo-EM and ATPase assays\nlimitations: Purified PMCA1 complex; subunit availability and other PMCA isoforms require separate evidence.\nresearch_relationship_category: regulation\ncompartment_description: Plasma membrane protein complex\n[ca-gong2018] Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin (2018). https://pubmed.ncbi.nlm.nih.gov/30190470/ DOI: 10.1038/s41467-018-06075-7","model_system":"Purified human PMCA1-neuroplastin complex; cryo-EM and ATPase assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ca-gong2018] Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin (2018). https://pubmed.ncbi.nlm.nih.gov/30190470/ DOI: 10.1038/s41467-018-06075-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}