{"id":"14fd577f-368c-5321-ba4f-219f700fe621","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-cndp2-ph","predicate":"hydrolyzes_conditionally","statement":"Human CNDP2 hydrolyzed carnosine only under alkaline assay conditions, with an optimum near pH 9.5.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"82355573-bc61-5fa2-9474-2d0b8751bfce","mechanism_event_label":"The similarly named enzyme behaved differently.","subject":{"id":"66621280-d7e5-5e8b-8ae1-873711628d87","slug":"human-cndp2","display_name":"Human cytosolic nonspecific dipeptidase / CNDP2","entity_type_key":"protein"},"object":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"82355573-bc61-5fa2-9474-2d0b8751bfce","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-cndp2-ph-event","event_type":"observed_relationship","label":"The similarly named enzyme behaved differently.","description":"Human CNDP2 hydrolyzed carnosine only under alkaline assay conditions, with an optimum near pH 9.5.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"66621280-d7e5-5e8b-8ae1-873711628d87","slug":"human-cndp2","display_name":"Human cytosolic nonspecific dipeptidase / CNDP2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"16a9848a-023b-5828-9fd1-e28b9eadb901","slug":"hydrogen-ion","display_name":"Hydrogen ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not assume CNDP2 is an equally active carnosinase at normal cytosolic pH.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The similarly named enzyme behaved differently.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12473676/ · DOI 10.1074/jbc.M209764200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2ce4805a-2213-5a95-92d1-630ca8dddbb8","evidence_kind":"source_excerpt","locator":"Lines 52-58","start_line":52,"end_line":58,"excerpt":"## carnosine-cndp2-ph\nThe similarly named enzyme behaved differently.\nHuman CNDP2 hydrolyzed carnosine only under alkaline assay conditions, with an optimum near pH 9.5.\nModel: Purified recombinant protein.\nLimitations: Do not assume CNDP2 is an equally active carnosinase at normal cytosolic pH.\nEvidence access: Primary abstract\nSequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12473676/ · DOI 10.1074/jbc.M209764200","model_system":"Purified recombinant protein.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"469cbc38203db56bd031f9d8a2dd2bfa387713950ca30199511cd137e927aa7a","revision_id":"cc409a15-8b04-50ee-a7ff-d1bf32f686bb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}