{"id":"14cb3e53-81b0-55a7-88da-b9b2b9ecccfd","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-u266-nac-rescue","predicate":"blocks","statement":"N-acetylcysteine blocked fisetin-induced apoptosis in human U266 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"95a0941f-9fb0-5f65-9a8c-a1068fda1373","mechanism_event_label":"An antioxidant intervention weakened the cell-killing response.","subject":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"object":{"id":"07297625-7ece-5fd3-aa02-66b83d0c7bae","slug":"human-u266-fisetin-apoptosis","display_name":"Fisetin-associated apoptosis in human U266 myeloma cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"95a0941f-9fb0-5f65-9a8c-a1068fda1373","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-u266-nac-rescue-event","event_type":"observed_relationship","label":"An antioxidant intervention weakened the cell-killing response.","description":"N-acetylcysteine blocked fisetin-induced apoptosis in human U266 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"07297625-7ece-5fd3-aa02-66b83d0c7bae","slug":"human-u266-fisetin-apoptosis","display_name":"Fisetin-associated apoptosis in human U266 myeloma cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Tumor-cell cotreatment experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish a clinical fisetin-NAC interaction or show whether oral exposures reproduce it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An antioxidant intervention weakened the cell-killing response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Activation of reactive oxygen species/AMP activated protein kinase signaling mediates fisetin-induced apoptosis in multiple myeloma U266 cells. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22261340/ · DOI 10.1016/j.canlet.2012.01.008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"21b3ac78-6c2e-5d2b-a42a-2b488b270819","evidence_kind":"source_excerpt","locator":"Lines 376-382","start_line":376,"end_line":382,"excerpt":"## fisetin-u266-nac-rescue\nAn antioxidant intervention weakened the cell-killing response.\nN-acetylcysteine blocked fisetin-induced apoptosis in human U266 cells.\nModel: Tumor-cell cotreatment experiment.\nLimitations: Does not establish a clinical fisetin-NAC interaction or show whether oral exposures reproduce it.\nEvidence access: Primary abstract\nActivation of reactive oxygen species/AMP activated protein kinase signaling mediates fisetin-induced apoptosis in multiple myeloma U266 cells. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22261340/ · DOI 10.1016/j.canlet.2012.01.008","model_system":"Tumor-cell cotreatment experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}