{"id":"14a5471c-d683-5333-82a6-3881c911f3db","stable_key":"research:blimp1-required-for-stat5-tfh-inhibition","predicate":"supports-inhibition-of","statement":"Blimp-1 deletion relieved STAT5-mediated inhibition of Tfh differentiation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"negative","is_public":true,"mechanism_event_id":"d3e27571-009d-5f99-b646-a059e5f82ac3","mechanism_event_label":"STAT5's inhibitory effect depended substantially on Blimp-1.","subject":{"id":"4a2427d7-9591-522f-a4fd-80641ade8f03","slug":"prdm1","display_name":"PRDM1","entity_type_key":"protein"},"object":{"id":"41da76dd-b500-58e8-bf3d-e2fa55fb8978","slug":"tfh-differentiation","display_name":"Follicular helper T-cell differentiation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d3e27571-009d-5f99-b646-a059e5f82ac3","stable_key":"research:blimp1-required-for-stat5-tfh-inhibition","event_type":"genetic-epistasis","label":"STAT5's inhibitory effect depended substantially on Blimp-1.","description":"Blimp-1 deletion relieved STAT5-mediated inhibition of Tfh differentiation.","status":"active","compartment":null,"participants":[{"entity":{"id":"4a2427d7-9591-522f-a4fd-80641ade8f03","slug":"prdm1","display_name":"PRDM1","entity_type_key":"protein"},"role":"required-regulator","stoichiometry":null,"state_label":"tested by deletion","sequence_order":0,"notes":""},{"entity":{"id":"d8de1e6a-fdad-52a0-8b9d-c9ea510a0238","slug":"stat5","display_name":"STAT5","entity_type_key":"protein_family"},"role":"upstream-perturbation","stoichiometry":null,"state_label":"constitutively active","sequence_order":1,"notes":""},{"entity":{"id":"41da76dd-b500-58e8-bf3d-e2fa55fb8978","slug":"tfh-differentiation","display_name":"Follicular helper T-cell differentiation","entity_type_key":"cellular_process"},"role":"rescued-process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"antigen-specific CD4 T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Conditional Prdm1 deletion with active STAT5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dependency does not prove direct STAT5 induction of PRDM1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0cdf0efa-ab30-5b94-a566-f6daa72b6426","evidence_kind":"curated_literature_summary","locator":"lines 942-952","start_line":942,"end_line":952,"excerpt":"## blimp1-required-for-stat5-tfh-inhibition\n\nSTAT5's inhibitory effect depended substantially on Blimp-1.\n\nBlimp-1 deletion relieved STAT5-mediated inhibition of Tfh differentiation.\n\nOrganism: mouse\nCell type: antigen-specific CD4 T cells\nExperimental model: Conditional Prdm1 deletion with active STAT5\nLimitations: Dependency does not prove direct STAT5 induction of PRDM1.\nPrimary reference: [STAT5 is a potent negative regulator of TFH cell differentiation](https://pmc.ncbi.nlm.nih.gov/articles/PMC3281266/)","model_system":"Conditional Prdm1 deletion with active STAT5","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"t-cell-fate","plain_language":"STAT5's inhibitory effect depended substantially on Blimp-1.","evidence_scope":"direct_experimental","papers":[{"key":"johnston-2012-stat5-tfh","title":"STAT5 is a potent negative regulator of TFH cell differentiation","url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC3281266/","doi":"10.1084/jem.20111174","year":2012,"model":"Mouse LCMV infection, transferred antigen-specific CD4 cells and genetic perturbations","summary":"Tests IL-2/STAT5 suppression of Tfh differentiation and its dependence on Blimp-1."}]}}