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(2016). https://pubmed.ncbi.nlm.nih.gov/27729528/ DOI: 10.1073/pnas.1608679113","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Naïve embryonic stem cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fc536419-bfaf-5df8-a48a-c12d44f4f57e","evidence_kind":"source_excerpt","locator":"Lines 1061-1073","start_line":1061,"end_line":1073,"excerpt":"### c-reg-tet2-rare-deletion\nDeleting a 104-bp intronic segment encompassing the mouse Tet2 retinoic-acid response element prevented the normal retinol-dependent Tet2 mRNA increase, supporting a cis-regulatory requirement.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The vitamin A response depended on a specific DNA control sequence in the Tet2 gene.\norganism: Mus musculus\ntissue_or_cell_type: Naïve embryonic stem cells\nexperimental_model: CRISPR Tet2 ΔRARE naïve ESCs compared with wild type\nlimitations: Deletion tests the segment rather than one isolated nucleotide; this is engineered machinery impairment, not vitamin A deficiency.\nexposure: Retinol titration as in Figure 3F; 104-bp deletion NCBI37 chr3:133197151–133197253.\ncross_nutrient: true\nevidence_location: Figure 3E–F\n[c-reg-hore] Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. 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