{"id":"1423fe9a-452d-57ac-9f0b-f3cc535c66a1","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-cathepsin-knockout","predicate":"not_required_for_release","statement":"Cathepsin-B-deficient macrophages retained crystal-induced IL-1 beta release in this study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"cc1fdc10-8a67-585f-b972-2619f9d92f47","mechanism_event_label":"Genetic loss did not reproduce the inhibitor-based necessity claim.","subject":{"id":"97540283-6cc6-58f8-9091-48b19d734612","slug":"mouse-ctsb","display_name":"Mouse cathepsin B","entity_type_key":"protein"},"object":{"id":"c17455e2-5805-55e7-9ecc-09c953115c84","slug":"mouse-il1b","display_name":"Mouse IL-1 beta","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"cc1fdc10-8a67-585f-b972-2619f9d92f47","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-cathepsin-knockout-event","event_type":"observed_relationship","label":"Genetic loss did not reproduce the inhibitor-based necessity claim.","description":"Cathepsin-B-deficient macrophages retained crystal-induced IL-1 beta release in this study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"97540283-6cc6-58f8-9091-48b19d734612","slug":"mouse-ctsb","display_name":"Mouse cathepsin B","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c17455e2-5805-55e7-9ecc-09c953115c84","slug":"mouse-il1b","display_name":"Mouse IL-1 beta","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b2d4231b-3893-5d4e-8b52-d0effdaa26c9","slug":"crystalline-silica","display_name":"Crystalline silica / quartz particles","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ae25f14e-2a54-5094-9952-a97b718f94a3","slug":"mouse-nlrp3","display_name":"Mouse NLRP3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse bone-marrow macrophages with silica/MSU comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Compensation and other cathepsins remain possible; no universal dismissal of lysosomal injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Genetic loss did not reproduce the inhibitor-based necessity claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Activation of NLRP3 inflammasome by crystalline structures via cell surface contact. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25445147/ · DOI 10.1038/srep07281","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"165b8a9a-6376-5d23-9988-71a66001ce47","evidence_kind":"source_excerpt","locator":"Lines 472-478","start_line":472,"end_line":478,"excerpt":"## silica-cathepsin-knockout\nGenetic loss did not reproduce the inhibitor-based necessity claim.\nCathepsin-B-deficient macrophages retained crystal-induced IL-1 beta release in this study.\nModel: Mouse bone-marrow macrophages with silica/MSU comparisons.\nLimitations: Compensation and other cathepsins remain possible; no universal dismissal of lysosomal injury.\nEvidence access: Primary full text\nActivation of NLRP3 inflammasome by crystalline structures via cell surface contact. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25445147/ · DOI 10.1038/srep07281","model_system":"Mouse bone-marrow macrophages with silica/MSU comparisons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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