{"id":"1404981f-d49e-5254-aa37-e57ec3429e80","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-mtor-ulk","predicate":"phosphorylates_to_disrupt","statement":"High mTOR activity phosphorylated Ulk1 Ser757 and disrupted its interaction with AMPK.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"194da2cd-468b-5081-b5d8-4389007e44c7","mechanism_event_label":"Nutrient sufficiency can restrain this initiation route.","subject":{"id":"9f025af3-f039-517f-908b-4af6fc65415e","slug":"mtorc1","display_name":"Mechanistic target of rapamycin complex 1","entity_type_key":"protein_complex"},"object":{"id":"b213f6fd-4968-5048-993c-b3bac2f8e908","slug":"ulk1-ampk-interaction","display_name":"Study-specific Ulk1–AMPK interaction","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"194da2cd-468b-5081-b5d8-4389007e44c7","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-mtor-ulk-event","event_type":"observed_relationship","label":"Nutrient sufficiency can restrain this initiation route.","description":"High mTOR activity phosphorylated Ulk1 Ser757 and disrupted its interaction with AMPK.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9f025af3-f039-517f-908b-4af6fc65415e","slug":"mtorc1","display_name":"Mechanistic target of rapamycin complex 1","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b213f6fd-4968-5048-993c-b3bac2f8e908","slug":"ulk1-ampk-interaction","display_name":"Study-specific Ulk1–AMPK interaction","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3b0658af-c665-51f7-8439-530c6da23aec","slug":"mouse-ulk1","display_name":"Mouse autophagy initiating kinase / Ulk1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8128eec0-61cc-51ef-8d4e-5ec9678e8dfa","slug":"ampk-complexes","display_name":"AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cellular kinase and autophagy assays; mouse Ulk1 numbering.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Specific signaling mechanism, not a whole-body autophagic-flux measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"Nutrient sufficiency can restrain this initiation route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21258367/ · DOI 10.1038/ncb2152","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"839cf816-2b0d-5a6e-9dd6-8b594458fc7f","evidence_kind":"source_excerpt","locator":"Lines 304-310","start_line":304,"end_line":310,"excerpt":"## fast-mtor-ulk\nNutrient sufficiency can restrain this initiation route.\nHigh mTOR activity phosphorylated Ulk1 Ser757 and disrupted its interaction with AMPK.\nModel: Cellular kinase and autophagy assays; mouse Ulk1 numbering.\nLimitations: Specific signaling mechanism, not a whole-body autophagic-flux measurement.\nEvidence access: Primary abstract\nAMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21258367/ · DOI 10.1038/ncb2152","model_system":"Cellular kinase and autophagy assays; mouse Ulk1 numbering.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"921d1eb5-2c88-52b9-9898-60b2b5bf6029","stable_key":"import-c9aa15a1-8ba2-5913-b2dd-372af304bf87","title":"Fasting: fuel switching, nutrient sensing, ketone signaling, nutrient dependencies and refeeding (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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