{"id":"13d25ac6-1cd2-5fe5-aa5e-540308602eb2","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-nac-rescue","predicate":"attenuates_tested","statement":"N-acetylcysteine at 1 mM attenuated aspartame-associated oxidative and barrier changes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6aaa1fd9-0163-5442-86d1-0d7b43af4947","mechanism_event_label":"An antioxidant-related perturbation changed the pathway response.","subject":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"object":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6aaa1fd9-0163-5442-86d1-0d7b43af4947","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-nac-rescue-event","event_type":"observed_relationship","label":"An antioxidant-related perturbation changed the pathway response.","description":"N-acetylcysteine at 1 mM attenuated aspartame-associated oxidative and barrier changes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"afffe733-49d5-55e9-8895-6e5e78d0f534","slug":"human-caco2-aspartame-ros","display_name":"Human Caco-2 ROS signal during aspartame exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2ccc9999-f1db-5fb7-b57c-513323c3e26e","slug":"cldn3","display_name":"Human claudin 3 / CLDN3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Figure 5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Caco-2 co-exposure experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that NAC supplements prevent effects of dietary aspartame.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An antioxidant-related perturbation changed the pathway response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Artificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7c0622d7-5122-55f4-a1a9-c855c60d98ea","evidence_kind":"source_excerpt","locator":"Lines 226-232","start_line":226,"end_line":232,"excerpt":"## aspartame-nac-rescue\nAn antioxidant-related perturbation changed the pathway response.\nN-acetylcysteine at 1 mM attenuated aspartame-associated oxidative and barrier changes.\nModel: Caco-2 co-exposure experiments.\nLimitations: Not evidence that NAC supplements prevent effects of dietary aspartame.\nEvidence access: Primary full text; Figure 5\nArtificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","model_system":"Caco-2 co-exposure experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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