{"id":"13bb631c-69b7-56ec-b173-dec71da76b11","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-glycine-substitution","predicate":"substitutes_in","statement":"Taurine-depleted cultured cells accumulated mitochondrial tRNA cmnm5U, whose glycine-derived group replaces the taurine-derived group of tau-m5U.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"564f9a7c-8359-5326-a588-b1f17908e176","mechanism_event_label":"Low taurine changed the chemical identity of the RNA modification.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"08d3afa7-7c04-5292-adbc-1b101df6c03f","slug":"mt-trna-cmnm5u","display_name":"Mitochondrial tRNA 5-carboxymethylaminomethyluridine / cmnm5U34","entity_type_key":"rna_modification"},"evidence_count":1,"mechanism_event":{"id":"564f9a7c-8359-5326-a588-b1f17908e176","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-glycine-substitution-event","event_type":"observed_relationship","label":"Low taurine changed the chemical identity of the RNA modification.","description":"Taurine-depleted cultured cells accumulated mitochondrial tRNA cmnm5U, whose glycine-derived group replaces the taurine-derived group of tau-m5U.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"08d3afa7-7c04-5292-adbc-1b101df6c03f","slug":"mt-trna-cmnm5u","display_name":"Mitochondrial tRNA 5-carboxymethylaminomethyluridine / cmnm5U34","entity_type_key":"rna_modification"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dd968147-5f93-56d5-bc83-7fa01f9b0e42","slug":"mt-trna-taurinomethyluridine","display_name":"Mitochondrial tRNA 5-taurinomethyluridine / tau-m5U34","entity_type_key":"rna_modification"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mass spectrometry of mitochondrial tRNAs from depleted cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Detection of an alternative mark does not establish complete functional rescue by glycine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Low taurine changed the chemical identity of the RNA modification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29390138/ · DOI 10.1093/nar/gky068","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"e3261035-4e91-548b-bfb0-83339fa8f03e","evidence_kind":"source_excerpt","locator":"Lines 241-247","start_line":241,"end_line":247,"excerpt":"## taurine-glycine-substitution\nLow taurine changed the chemical identity of the RNA modification.\nTaurine-depleted cultured cells accumulated mitochondrial tRNA cmnm5U, whose glycine-derived group replaces the taurine-derived group of tau-m5U.\nModel: Mass spectrometry of mitochondrial tRNAs from depleted cells.\nLimitations: Detection of an alternative mark does not establish complete functional rescue by glycine.\nEvidence access: Primary abstract\nMetabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29390138/ · DOI 10.1093/nar/gky068","model_system":"Mass spectrometry of mitochondrial tRNAs from depleted cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}