{"id":"139c68c5-bb29-5ef6-9b8b-031c0ca06428","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:b12-bovine-ado-to-methyl","predicate":"is-processed-into","statement":"Bovine aortic endothelial cells converted cobalt-57-labeled adenosylcobalamin into labeled methylcobalamin during metabolic labeling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"570a6830-0dda-5ddc-b711-ac92a260291a","mechanism_event_label":"These bovine cells could rework one active B12 form into the other.","subject":{"id":"07657685-74aa-5d99-8625-0f6a3b521f07","slug":"adenosylcobalamin","display_name":"5'-Deoxyadenosylcobalamin","entity_type_key":"small_molecule"},"object":{"id":"6563fbad-ebdc-5408-966a-d24f8b8cd854","slug":"methylcobalamin","display_name":"Methylcobalamin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"570a6830-0dda-5ddc-b711-ac92a260291a","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:b12-bovine-ado-to-methyl-event","event_type":"biochemical_relationship","label":"These bovine cells could rework one active B12 form into the other.","description":"Bovine aortic endothelial cells converted cobalt-57-labeled adenosylcobalamin into labeled methylcobalamin during metabolic labeling.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"07657685-74aa-5d99-8625-0f6a3b521f07","slug":"adenosylcobalamin","display_name":"5'-Deoxyadenosylcobalamin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6563fbad-ebdc-5408-966a-d24f8b8cd854","slug":"methylcobalamin","display_name":"Methylcobalamin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract; 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topical membership is not evidence of a direct dietary effect.\nplain_language: These bovine cells could rework one active B12 form into the other.\norganism: Bos taurus\ntissue_or_cell_type: Aortic endothelium\nexperimental_model: Bovine aortic endothelial cell metabolic labeling\nlimitations: Cellular conversion supports processing but does not by itself identify every enzyme or establish a human supplement hierarchy.\nexposure: 0.125 nM labeled alkylcobalamin; 48-hour processing assay protected from light\ncross_nutrient: false\nevidence_location: Abstract; indexed Methods: Assessment of dealkylation; Discussion\n[hannibal-2009-cell-processing] Processing of alkylcobalamins in mammalian cells: A role for the MMACHC (cblC) gene product (2009). https://pubmed.ncbi.nlm.nih.gov/19447654/ DOI: 10.1016/j.ymgme.2009.04.005","model_system":"Bovine aortic endothelial cell metabolic labeling","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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