{"id":"139af364-5135-5109-b958-8a47867a34cc","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-truncated-ctr1-endosome","predicate":"mobilizes","statement":"The truncated Ctr1 form mobilized endosomal copper stores, whereas full-length Ctr1 efficiently supported plasma-membrane uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"409fc3c3-7c64-52af-8b64-56541b466dad","mechanism_event_label":"Copper trapped inside a compartment needs a different access step.","subject":{"id":"89ff6a87-8777-5d3c-99be-68eac76e3e05","slug":"mouse-ctr1-truncated","display_name":"Mouse Ctr1 with cleaved extracellular N-terminal domain","entity_type_key":"protein_state"},"object":{"id":"d7eca9d5-5d71-5564-93d1-bdf69fd7ab90","slug":"endosomal-copper-mobilization","display_name":"Mobilization of copper from endosomal stores","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"409fc3c3-7c64-52af-8b64-56541b466dad","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-truncated-ctr1-endosome-event","event_type":"biochemical_relationship","label":"Copper trapped inside a compartment needs a different access step.","description":"The truncated Ctr1 form mobilized endosomal copper stores, whereas full-length Ctr1 efficiently supported plasma-membrane uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9b443d47-b3cc-538d-ad34-e93b5c7880c8","slug":"mouse-slc31a1","display_name":"Mouse copper transporter Ctr1 / Slc31a1","entity_type_key":"protein"},"role":"full-length comparator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89ff6a87-8777-5d3c-99be-68eac76e3e05","slug":"mouse-ctr1-truncated","display_name":"Mouse Ctr1 with cleaved extracellular N-terminal domain","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d7eca9d5-5d71-5564-93d1-bdf69fd7ab90","slug":"endosomal-copper-mobilization","display_name":"Mobilization of copper from endosomal stores","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/copper-research/24167251.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7315e9041dddd5510d6efd970560c80316d38120ba1d8eefca10952be8ea0968\", \"start_char\": 0, \"end_char\": 1212, \"text_sha256\": \"7315e9041dddd5510d6efd970560c80316d38120ba1d8eefca10952be8ea0968\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ctr2 knockout and Ctr1 processing experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Ctr2 deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"CTR2 regulates truncated CTR1 and intracellular mobilization; it should not simply be treated as a duplicate plasma-membrane CTR1 importer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Copper research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Copper trapped inside a compartment needs a different access step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[copper-p24167251] Ctr2 regulates biogenesis of a cleaved form of mammalian Ctr1 metal transporter lacking the copper- and cisplatin-binding ecto-domain. (2013). https://pubmed.ncbi.nlm.nih.gov/24167251/ DOI: 10.1073/pnas.1311749110","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Endosomal stores versus plasma-membrane uptake","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"459ad5c2-005e-537e-9650-e93b33efeade","evidence_kind":"source_excerpt","locator":"Lines 481-492","start_line":481,"end_line":492,"excerpt":"### copper-truncated-ctr1-endosome\nThe truncated Ctr1 form mobilized endosomal copper stores, whereas full-length Ctr1 efficiently supported plasma-membrane uptake.\nCondition category: normal\nnutrient_topic: Copper research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Copper trapped inside a compartment needs a different access step.\norganism: Mouse\ntissue_or_cell_type: Endosomal stores versus plasma-membrane uptake\nexperimental_model: Ctr2 knockout and Ctr1 processing experiments\nlimitations: CTR2 regulates truncated CTR1 and intracellular mobilization; it should not simply be treated as a duplicate plasma-membrane CTR1 importer.\nexposure: Ctr2 deletion\nevidence_span: {\"source_cache\": \"artifacts/copper-research/24167251.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7315e9041dddd5510d6efd970560c80316d38120ba1d8eefca10952be8ea0968\", \"start_char\": 0, \"end_char\": 1212, \"text_sha256\": \"7315e9041dddd5510d6efd970560c80316d38120ba1d8eefca10952be8ea0968\"}\n[copper-p24167251] Ctr2 regulates biogenesis of a cleaved form of mammalian Ctr1 metal transporter lacking the copper- and cisplatin-binding ecto-domain. (2013). https://pubmed.ncbi.nlm.nih.gov/24167251/ DOI: 10.1073/pnas.1311749110","model_system":"Ctr2 knockout and Ctr1 processing experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [copper-p24167251] Ctr2 regulates biogenesis of a cleaved form of mammalian Ctr1 metal transporter lacking the copper- and cisplatin-binding ecto-domain. (2013). https://pubmed.ncbi.nlm.nih.gov/24167251/ DOI: 10.1073/pnas.1311749110","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9afba495-cbdc-51aa-998e-70a930dba3be","stable_key":"import-0ad8610d-d575-5870-b7cd-763a9f750783","title":"Copper: transport, cuproenzymes, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"84b0f62b2dae6835fa26902be87625c003c6c707492d3007e8f9d15420669008","revision_id":"d7e35b8b-3f77-56d9-90b5-5f542c63f321","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}