{"id":"139501c8-09a1-590f-9f0e-255f3e25878a","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-binding-water-model","predicate":"has_proposed_water_mediated_contacts_with","statement":"The model proposed two pocket waters bridging aspartame carbonyls to D142 and L279.","claim_class":"hypothesis_link","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"34c359b2-e9c8-5b0f-8d89-77e7c5f24f60","mechanism_event_label":"Water-mediated contacts are a testable structural explanation.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"09713aab-d5e3-589c-83c2-95d3989efd62","slug":"tas1r2","display_name":"Human taste receptor TAS1R2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"34c359b2-e9c8-5b0f-8d89-77e7c5f24f60","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-binding-water-model-event","event_type":"observed_relationship","label":"Water-mediated contacts are a testable structural explanation.","description":"The model proposed two pocket waters bridging aspartame carbonyls to D142 and L279.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"09713aab-d5e3-589c-83c2-95d3989efd62","slug":"tas1r2","display_name":"Human taste receptor TAS1R2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7ab6f209-2089-5008-b64e-64f8db8c7771","slug":"tas1r3","display_name":"Human taste receptor TAS1R3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Docking refined with functional mutagenesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Proposed molecular model, not a directly resolved bound-water structure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Water-mediated contacts are a testable structural explanation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Characterization of the Binding Site of Aspartame in the Human Sweet Taste Receptor. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26377607/ · DOI 10.1093/chemse/bjv045","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"934df298-8c4a-52be-97d0-0eac9c360c7d","evidence_kind":"source_excerpt","locator":"Lines 178-184","start_line":178,"end_line":184,"excerpt":"## aspartame-binding-water-model\nWater-mediated contacts are a testable structural explanation.\nThe model proposed two pocket waters bridging aspartame carbonyls to D142 and L279.\nModel: Docking refined with functional mutagenesis.\nLimitations: Proposed molecular model, not a directly resolved bound-water structure.\nEvidence access: Primary abstract\nCharacterization of the Binding Site of Aspartame in the Human Sweet Taste Receptor. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26377607/ · DOI 10.1093/chemse/bjv045","model_system":"Docking refined with functional mutagenesis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}