{"id":"1375130e-1aa0-5ab5-955c-122a9eb5b34a","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-endothelial-no","predicate":"increases","statement":"Agmatine increased nitrite production about threefold in bovine endothelial cells; idazoxan inhibited the effect whereas yohimbine did not.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6ba7a1bc-5264-5585-b6b5-0453aa819438","mechanism_event_label":"An intact-cell signaling effect can differ from direct enzyme inhibition.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"450a764c-3606-5e69-a0b5-bc6eb120282d","slug":"bovine-endothelial-nitrite-output","display_name":"Bovine pulmonary-endothelial nitrite output","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6ba7a1bc-5264-5585-b6b5-0453aa819438","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-endothelial-no-event","event_type":"observed_relationship","label":"An intact-cell signaling effect can differ from direct enzyme inhibition.","description":"Agmatine increased nitrite production about threefold in bovine endothelial cells; 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pharmacological antagonist comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The imidazoline-associated mechanism is pharmacologically inferred; a specific receptor gene was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An intact-cell signaling effect can differ from direct enzyme inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine activation of nitric oxide synthase in endothelial cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9010916/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8e115612-bbda-5ce4-9766-a9b43db57e33","evidence_kind":"source_excerpt","locator":"Lines 252-258","start_line":252,"end_line":258,"excerpt":"## agmatine-sulfate-endothelial-no\nAn intact-cell signaling effect can differ from direct enzyme inhibition.\nAgmatine increased nitrite production about threefold in bovine endothelial cells; idazoxan inhibited the effect whereas yohimbine did not.\nModel: Bovine endothelial culture; pharmacological antagonist comparison.\nLimitations: The imidazoline-associated mechanism is pharmacologically inferred; a specific receptor gene was not established.\nEvidence access: Primary abstract\nAgmatine activation of nitric oxide synthase in endothelial cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9010916/","model_system":"Bovine endothelial culture; pharmacological antagonist comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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