{"id":"1359910c-3405-5037-b2bb-3499f85befb3","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-mfsd12-cysteine","predicate":"supports","statement":"Lysosomes isolated from human HEK293T cells with MFSD12 accumulated more labeled cysteine than lysosomes from MFSD12-knockout cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e15a355d-a134-558c-830e-bf9cb22292d6","mechanism_event_label":"Pigment-related machinery also handles another amino acid in non-pigmented cells.","subject":{"id":"aa988fc7-98e8-5333-8742-6e3d9f74a5d6","slug":"mfsd12","display_name":"Human MFSD12 cysteine transport component","entity_type_key":"protein"},"object":{"id":"758687d5-f3db-593b-a2f9-432c696c6ae8","slug":"human-lysosomal-cysteine-import","display_name":"Human lysosomal cysteine import","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e15a355d-a134-558c-830e-bf9cb22292d6","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-mfsd12-cysteine-event","event_type":"observed_relationship","label":"Pigment-related machinery also handles another amino acid in non-pigmented cells.","description":"Lysosomes isolated from human HEK293T cells with MFSD12 accumulated more labeled cysteine than lysosomes from MFSD12-knockout cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aa988fc7-98e8-5333-8742-6e3d9f74a5d6","slug":"mfsd12","display_name":"Human MFSD12 cysteine transport component","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"758687d5-f3db-593b-a2f9-432c696c6ae8","slug":"human-lysosomal-cysteine-import","display_name":"Human lysosomal cysteine import","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Fig. 4 and transport methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HEK293T isolated lysosomes; radiolabeled cysteine transport.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is lysosomal import; the separate pigment-cell findings connect the same protein to melanosomes. Purified-protein sufficiency was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Pigment-related machinery also handles another amino acid in non-pigmented cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"MFSD12 mediates the import of cysteine into melanosomes and lysosomes. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33208952/ · DOI 10.1038/s41586-020-2937-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"718093d8-c1a2-5dd4-8634-0089a06fd8fc","evidence_kind":"source_excerpt","locator":"Lines 172-178","start_line":172,"end_line":178,"excerpt":"## l-tyrosine-mfsd12-cysteine\nPigment-related machinery also handles another amino acid in non-pigmented cells.\nLysosomes isolated from human HEK293T cells with MFSD12 accumulated more labeled cysteine than lysosomes from MFSD12-knockout cells.\nModel: Human HEK293T isolated lysosomes; radiolabeled cysteine transport.\nLimitations: This is lysosomal import; the separate pigment-cell findings connect the same protein to melanosomes. Purified-protein sufficiency was not established.\nEvidence access: Primary full text; Fig. 4 and transport methods\nMFSD12 mediates the import of cysteine into melanosomes and lysosomes. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33208952/ · DOI 10.1038/s41586-020-2937-x","model_system":"Human HEK293T isolated lysosomes; radiolabeled cysteine transport.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7777f4440a8bfb14a04ff73e73392ccc45d8ee89f7853ecbcc0027d6f563b8a0","revision_id":"e1206111-3a75-5809-b8e9-231ff809ff1e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}