{"id":"134f50eb-3269-53dc-ace0-43449553de33","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-yars2-charging","predicate":"charges","statement":"Recombinant human YARS2 supported tyrosyl-tRNA aminoacylation; the F52L variant retained activity with abnormal kinetics.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"79d526fe-5b70-54a5-9e9d-4e150da6838a","mechanism_event_label":"Mitochondria use a separate tyrosine-loading enzyme.","subject":{"id":"91ccef7b-443e-5a1a-9656-adc2d867523f","slug":"yars2","display_name":"Human mitochondrial tyrosyl-tRNA synthetase / YARS2","entity_type_key":"protein"},"object":{"id":"f1462b10-3fd3-5a9d-9e30-8b48a35929a5","slug":"human-mitochondrial-tyrosyl-trna","display_name":"Human mitochondrial Tyr-tRNA Tyr","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"79d526fe-5b70-54a5-9e9d-4e150da6838a","stable_key":"63ce713e-6aea-59f6-9896-ca30e010b2ce:l-tyrosine-yars2-charging-event","event_type":"observed_relationship","label":"Mitochondria use a separate tyrosine-loading enzyme.","description":"Recombinant human YARS2 supported tyrosyl-tRNA aminoacylation; the F52L variant retained activity with abnormal kinetics.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"91ccef7b-443e-5a1a-9656-adc2d867523f","slug":"yars2","display_name":"Human mitochondrial tyrosyl-tRNA synthetase / YARS2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f1462b10-3fd3-5a9d-9e30-8b48a35929a5","slug":"human-mitochondrial-tyrosyl-trna","display_name":"Human mitochondrial Tyr-tRNA Tyr","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wild-type and F52L human enzyme aminoacylation assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Retained activity does not mean normal function; this does not establish a tyrosine-rescue dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Tyrosine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Mitochondria use a separate tyrosine-loading enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mutation of the mitochondrial tyrosyl-tRNA synthetase gene, YARS2, causes myopathy, lactic acidosis, and sideroblastic anemia--MLASA syndrome. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20598274/ · DOI 10.1016/j.ajhg.2010.06.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"851c1fae-38ac-54d6-a938-9e84fd58d6f4","evidence_kind":"source_excerpt","locator":"Lines 76-82","start_line":76,"end_line":82,"excerpt":"## l-tyrosine-yars2-charging\nMitochondria use a separate tyrosine-loading enzyme.\nRecombinant human YARS2 supported tyrosyl-tRNA aminoacylation; the F52L variant retained activity with abnormal kinetics.\nModel: Wild-type and F52L human enzyme aminoacylation assays.\nLimitations: Retained activity does not mean normal function; this does not establish a tyrosine-rescue dose.\nEvidence access: Primary abstract\nMutation of the mitochondrial tyrosyl-tRNA synthetase gene, YARS2, causes myopathy, lactic acidosis, and sideroblastic anemia--MLASA syndrome. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20598274/ · DOI 10.1016/j.ajhg.2010.06.001","model_system":"Wild-type and F52L human enzyme aminoacylation assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"12917df2-c6e0-5b61-850f-dbff6d4b4d30","stable_key":"import-63ce713e-6aea-59f6-9896-ca30e010b2ce","title":"L-Tyrosine: catecholamines, thyroid chemistry, pigment, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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