{"id":"126e9c47-85a7-5fd5-8e74-6a1725c8d9e1","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-charging-fidelity","predicate":"edits_mischarging_with","statement":"Human cytosolic and mitochondrial phenylalanyl-tRNA synthetases could mischarge L-DOPA in biochemical assays; cytosolic editing hydrolyzed L-DOPA- and tyrosine-mischarged tRNA, whereas the mitochondrial system discriminated less effectively.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3dfd3661-bd72-5aff-af42-3892e789b880","mechanism_event_label":"Similar-looking molecules can challenge protein-building accuracy.","subject":{"id":"26dc1ace-5c76-55b6-b3c8-b48b3f4b4c8e","slug":"farsa-farsb","display_name":"Human cytosolic FARSA/FARSB phenylalanyl-tRNA synthetase","entity_type_key":"protein_complex"},"object":{"id":"d2e54a66-7f3c-5bcc-adbe-9f66ad4264f4","slug":"l-dopa","display_name":"L-3,4-Dihydroxyphenylalanine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"3dfd3661-bd72-5aff-af42-3892e789b880","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-charging-fidelity-event","event_type":"observed_relationship","label":"Similar-looking molecules can challenge protein-building accuracy.","description":"Human cytosolic and mitochondrial phenylalanyl-tRNA synthetases could mischarge L-DOPA in biochemical assays; cytosolic editing hydrolyzed L-DOPA- and tyrosine-mischarged tRNA, whereas the mitochondrial system discriminated less effectively.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"26dc1ace-5c76-55b6-b3c8-b48b3f4b4c8e","slug":"farsa-farsb","display_name":"Human cytosolic FARSA/FARSB phenylalanyl-tRNA synthetase","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d2e54a66-7f3c-5bcc-adbe-9f66ad4264f4","slug":"l-dopa","display_name":"L-3,4-Dihydroxyphenylalanine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"947f3409-8344-5cd6-867e-e1d6172d6043","slug":"fars2","display_name":"Human mitochondrial phenylalanyl-tRNA synthetase / FARS2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bcfef85f-831d-5439-ba51-1aef4b090441","slug":"l-tyrosine","display_name":"L-Tyrosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In-vitro human cytosolic and mitochondrial synthetase assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay mischarging does not establish clinical proteotoxicity from a usual phenylalanine intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Similar-looking molecules can challenge protein-building accuracy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bacterial and eukaryotic phenylalanyl-tRNA synthetases catalyze misaminoacylation of tRNA(Phe) with 3,4-dihydroxy-L-phenylalanine. · 2011 · https://pubmed.ncbi.nlm.nih.gov/22035791/ · DOI 10.1016/j.chembiol.2011.08.008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2b05935f-e312-503a-9053-fc2ef9384d06","evidence_kind":"source_excerpt","locator":"Lines 134-140","start_line":134,"end_line":140,"excerpt":"## l-phenylalanine-charging-fidelity\nSimilar-looking molecules can challenge protein-building accuracy.\nHuman cytosolic and mitochondrial phenylalanyl-tRNA synthetases could mischarge L-DOPA in biochemical assays; cytosolic editing hydrolyzed L-DOPA- and tyrosine-mischarged tRNA, whereas the mitochondrial system discriminated less effectively.\nModel: In-vitro human cytosolic and mitochondrial synthetase assays.\nLimitations: Assay mischarging does not establish clinical proteotoxicity from a usual phenylalanine intake.\nEvidence access: Primary abstract\nBacterial and eukaryotic phenylalanyl-tRNA synthetases catalyze misaminoacylation of tRNA(Phe) with 3,4-dihydroxy-L-phenylalanine. · 2011 · https://pubmed.ncbi.nlm.nih.gov/22035791/ · DOI 10.1016/j.chembiol.2011.08.008","model_system":"In-vitro human cytosolic and mitochondrial synthetase assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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