{"id":"123a4d05-dc17-526e-b0d4-06905e1b865a","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-urine-cysgly","predicate":"was_recovered_in_urine_as","statement":"Sulforaphane-cysteinylglycine was separately quantified in human urine after broccoli sprout exposure.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"48fb3911-c752-559e-8555-c99f8273dc09","mechanism_event_label":"This metabolite has its own identity and can be measured separately from the parent.","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"025c36d0-d256-5649-9d01-84e69625fd4f","slug":"sulforaphane-cysteinylglycine","display_name":"Sulforaphane-cysteinylglycine conjugate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"48fb3911-c752-559e-8555-c99f8273dc09","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-urine-cysgly-event","event_type":"observed_intervention","label":"This metabolite has its own identity and can be measured separately from the parent.","description":"Sulforaphane-cysteinylglycine was separately quantified in human urine after broccoli sprout exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"025c36d0-d256-5649-9d01-84e69625fd4f","slug":"sulforaphane-cysteinylglycine","display_name":"Sulforaphane-cysteinylglycine conjugate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/18729326.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3\", \"start_char\": 0, \"end_char\": 2172, \"text_sha256\": \"1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isotope-dilution LC-MS/MS assay validation in intervention urines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Broccoli sprout intervention specimens with equivalent administered preparation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Analytical exposure study; metabolite concentration is not tissue efficacy and does not identify each responsible human enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human, 98 urine specimens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"This metabolite has its own identity and can be measured separately from the parent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p18729326] Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry. (2008). https://pubmed.ncbi.nlm.nih.gov/18729326/ DOI: 10.1021/tx800210k","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Urinary mercapturic-acid pathway species","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d126421b-38df-5ddd-a202-7eedcce428f7","evidence_kind":"source_excerpt","locator":"Lines 346-357","start_line":346,"end_line":357,"excerpt":"### sulforaphane-urine-cysgly\nSulforaphane-cysteinylglycine was separately quantified in human urine after broccoli sprout exposure.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: This metabolite has its own identity and can be measured separately from the parent.\norganism: Human, 98 urine specimens\ntissue_or_cell_type: Urinary mercapturic-acid pathway species\nexperimental_model: Isotope-dilution LC-MS/MS assay validation in intervention urines\nlimitations: Analytical exposure study; metabolite concentration is not tissue efficacy and does not identify each responsible human enzyme.\nexposure: Broccoli sprout intervention specimens with equivalent administered preparation\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/18729326.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3\", \"start_char\": 0, \"end_char\": 2172, \"text_sha256\": \"1b30293ede979c158852939b48f68a2cd71fa605bbca813a9fe2325f99e5c1d3\"}\n[sulforaphane-p18729326] Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry. (2008). https://pubmed.ncbi.nlm.nih.gov/18729326/ DOI: 10.1021/tx800210k","model_system":"Isotope-dilution LC-MS/MS assay validation in intervention urines","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sulforaphane-p18729326] Quantification of sulforaphane mercapturic acid pathway conjugates in human urine by high-performance liquid chromatography and isotope-dilution tandem mass spectrometry. 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