{"id":"11f4bd54-d7bf-5763-9805-8ec7aa8a2a21","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-tlr2-mutant","predicate":"reduces_binding_of","statement":"DM7 binding was lower to R315Q/R316Q/R321Q/K347Q TLR2 than to the R321Q/K347Q construct.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d814e5dd-3015-582a-a106-e9d09bf447c6","mechanism_event_label":"Changing the receptor charges weakens the measured interaction.","subject":{"id":"64fb9c7e-065e-50dd-a88b-a067413b978a","slug":"human-tlr2-qqqq","display_name":"Human TLR2 R315Q/R316Q/R321Q/K347Q construct","entity_type_key":"protein_state"},"object":{"id":"d54fb89f-e04d-580a-aff2-a97bdab74c19","slug":"low-dm-lemon-pectin","display_name":"Low-methylesterified lemon pectin, DM7 preparation","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"d814e5dd-3015-582a-a106-e9d09bf447c6","stable_key":"d17caac5-7018-5ca8-9e96-95afeb384bc4:pectin-tlr2-mutant-event","event_type":"biochemical_relationship","label":"Changing the receptor charges weakens the measured interaction.","description":"DM7 binding was lower to R315Q/R316Q/R321Q/K347Q TLR2 than to the R321Q/K347Q construct.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7d88fac7-eee3-541d-a202-e73c3f04186e","slug":"tlr2","display_name":"Human Toll-like receptor 2 / TLR2","entity_type_key":"protein"},"role":"affected_receptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b37018a6-bfc3-52d0-885f-8a5e441e8e75","slug":"human-tlr2-rrqq","display_name":"Human TLR2 R321Q/K347Q construct","entity_type_key":"protein_state"},"role":"comparator","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"64fb9c7e-065e-50dd-a88b-a067413b978a","slug":"human-tlr2-qqqq","display_name":"Human TLR2 R315Q/R316Q/R321Q/K347Q construct","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d54fb89f-e04d-580a-aff2-a97bdab74c19","slug":"low-dm-lemon-pectin","display_name":"Low-methylesterified lemon pectin, DM7 preparation","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/pectin-research/29545800.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4b9e856ec2420c457b98f455ec243b742525b63d9382b69ef087456e0136cb42\", \"start_char\": 37198, \"end_char\": 38286, \"text_sha256\": \"1e7101498d68b59ef3b4b7d5ad57ce9d4aa25f535bc5000e421099adc8720b19\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor binding, reporter cells, human dendritic cells and mouse ileitis model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lemon pectin DM7 versus DM75; cell exposures 1-100 micrograms/mL; mice 3 mg/day before doxorubicin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Model-specific. 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Binding and cytokine experiments do not establish prevention of human inflammatory disease. Lack of increased measured SCFAs does not exclude every microbial contribution.\nexposure: Lemon pectin DM7 versus DM75; cell exposures 1-100 micrograms/mL; mice 3 mg/day before doxorubicin\nevidence_span: {\"source_cache\": \"artifacts/pectin-research/29545800.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4b9e856ec2420c457b98f455ec243b742525b63d9382b69ef087456e0136cb42\", \"start_char\": 37198, \"end_char\": 38286, \"text_sha256\": \"1e7101498d68b59ef3b4b7d5ad57ce9d4aa25f535bc5000e421099adc8720b19\"}\n[pectin-p29545800] Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2-1 and Prevents Doxorubicin-Induced Ileitis. 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