{"id":"11dc1afc-0f10-5af3-999f-98d64035c3b0","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-sample-hydrolysis","predicate":"affects_measurement","statement":"Intact carnosine became detectable in sampled plasma when ex vivo blood carnosinase activity was inhibited.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4f63c998-0852-5d28-b9c1-5aa5b824126f","mechanism_event_label":"Breakdown after blood collection can hide the signal.","subject":{"id":"4c1f9be1-accc-547e-a1fe-a1f824118a89","slug":"human-cndp1","display_name":"Human carnosinase 1 / CNDP1","entity_type_key":"protein"},"object":{"id":"96ee8c6c-71cb-5130-8723-f7a9c8d65d2c","slug":"human-plasma-carnosine-measurement","display_name":"Human measured plasma intact carnosine","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4f63c998-0852-5d28-b9c1-5aa5b824126f","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-sample-hydrolysis-event","event_type":"observed_relationship","label":"Breakdown after blood collection can hide the signal.","description":"Intact carnosine became detectable in sampled plasma when ex vivo blood carnosinase activity was inhibited.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4c1f9be1-accc-547e-a1fe-a1f824118a89","slug":"human-cndp1","display_name":"Human carnosinase 1 / CNDP1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"96ee8c6c-71cb-5130-8723-f7a9c8d65d2c","slug":"human-plasma-carnosine-measurement","display_name":"Human measured plasma intact carnosine","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human blood-sample handling experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A negative untreated plasma assay cannot prove no absorption occurred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Breakdown after blood collection can hide the signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Intestinal absorption of the intact peptide carnosine in man, and comparison with intestinal permeability to lactulose. · 1991 · https://pubmed.ncbi.nlm.nih.gov/1910085/ · DOI 10.1113/jphysiol.1991.sp018673","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; 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primary-abstract references and experimental limitations individually identified. 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