{"id":"1175fd5d-017a-517b-b74c-4df094785a51","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-kupffer-chloride-dependence","predicate":"enables_tested","statement":"Chloride-free buffer abolished the reported glycine effect, and 1 micromolar strychnine reversed it; glycine hyperpolarized the tested Kupffer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a71cbcac-5ad2-50be-a255-b753248b3cf1","mechanism_event_label":"The ionic environment was required for the observed response.","subject":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"object":{"id":"6d316e3b-ba0b-5161-a57e-59c2e3869810","slug":"rat-kupffer-glycine-chloride-response","display_name":"Chloride-dependent glycine response in rat Kupffer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a71cbcac-5ad2-50be-a255-b753248b3cf1","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-kupffer-chloride-dependence-event","event_type":"observed_relationship","label":"The ionic environment was required for the observed response.","description":"Chloride-free buffer abolished the reported glycine effect, and 1 micromolar strychnine reversed it; glycine hyperpolarized the tested Kupffer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6d316e3b-ba0b-5161-a57e-59c2e3869810","slug":"rat-kupffer-glycine-chloride-response","display_name":"Chloride-dependent glycine response in rat Kupffer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat immune-cell ion substitution and antagonist experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacology does not uniquely identify a receptor-subunit combination; high-dose strychnine behaved differently.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The ionic environment was required for the observed response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Kupffer cells contain a glycine-gated chloride channel. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9227496/ · DOI 10.1152/ajpgi.1997.272.6.G1581","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eaf03f43-69d1-532d-a119-bafa95daa49f","evidence_kind":"source_excerpt","locator":"Lines 338-344","start_line":338,"end_line":344,"excerpt":"## glycine-kupffer-chloride-dependence\nThe ionic environment was required for the observed response.\nChloride-free buffer abolished the reported glycine effect, and 1 micromolar strychnine reversed it; glycine hyperpolarized the tested Kupffer cells.\nModel: Rat immune-cell ion substitution and antagonist experiments.\nLimitations: Pharmacology does not uniquely identify a receptor-subunit combination; high-dose strychnine behaved differently.\nEvidence access: Primary abstract\nKupffer cells contain a glycine-gated chloride channel. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9227496/ · DOI 10.1152/ajpgi.1997.272.6.G1581","model_system":"Rat immune-cell ion substitution and antagonist experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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