{"id":"11716d39-c0cf-536d-a3d2-e6ef2b83633f","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-macrophage-gsh","predicate":"supplies_glycine_for","statement":"Serine-derived glycine supported glutathione synthesis required for optimal LPS-induced IL-1beta mRNA expression; inflammasome activation was not the affected step.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f297e345-1dfe-52ae-8999-c1b89a0abc54","mechanism_event_label":"An antioxidant-building pathway can support inflammatory gene expression.","subject":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"object":{"id":"142ac8f2-c0bc-58a6-945f-0d62dcc14973","slug":"mouse-macrophage-gsh-il1b","display_name":"Glutathione-dependent IL-1beta transcription in mouse macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f297e345-1dfe-52ae-8999-c1b89a0abc54","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-macrophage-gsh-event","event_type":"observed_relationship","label":"An antioxidant-building pathway can support inflammatory gene expression.","description":"Serine-derived glycine supported glutathione synthesis required for optimal LPS-induced IL-1beta mRNA expression; inflammasome activation was not the affected step.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"142ac8f2-c0bc-58a6-945f-0d62dcc14973","slug":"mouse-macrophage-gsh-il1b","display_name":"Glutathione-dependent IL-1beta transcription in mouse macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse macrophage metabolic manipulation and LPS stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"IL-1beta transcription and inflammasome-mediated processing are different endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Serine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An antioxidant-building pathway can support inflammatory gene expression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Serine Metabolism Supports Macrophage IL-1β Production. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30773464/ · DOI 10.1016/j.cmet.2019.01.014","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"909adbab-1286-5b55-817d-8aaf722fd54d","evidence_kind":"source_excerpt","locator":"Lines 230-236","start_line":230,"end_line":236,"excerpt":"## l-serine-macrophage-gsh\nAn antioxidant-building pathway can support inflammatory gene expression.\nSerine-derived glycine supported glutathione synthesis required for optimal LPS-induced IL-1beta mRNA expression; inflammasome activation was not the affected step.\nModel: Mouse macrophage metabolic manipulation and LPS stimulation.\nLimitations: IL-1beta transcription and inflammasome-mediated processing are different endpoints.\nEvidence access: Primary abstract\nSerine Metabolism Supports Macrophage IL-1β Production. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30773464/ · DOI 10.1016/j.cmet.2019.01.014","model_system":"Mouse macrophage metabolic manipulation and LPS stimulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70bcf57a-36bb-563f-9073-2d616e5f155a","stable_key":"import-649e861b-265a-5912-bc36-3a73e53ef892","title":"L-Serine: synthesis, one-carbon metabolism, lipids and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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