{"id":"11687794-2f66-5377-a1d0-0831be51e7a4","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-endothelial-calcium","predicate":"induces","statement":"Agmatine induced cytosolic calcium transients in bovine pulmonary artery endothelial cells; repeated exposure downregulated the response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"514a3d3e-1f1c-5b1f-9328-635eac2478f8","mechanism_event_label":"The compound can change the activating signal upstream of an enzyme.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"3b6f7c08-a355-540a-a894-685aac98c953","slug":"bovine-pulmonary-endothelial-calcium-transients","display_name":"Bovine pulmonary-endothelial cytosolic calcium transients","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"514a3d3e-1f1c-5b1f-9328-635eac2478f8","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-endothelial-calcium-event","event_type":"observed_relationship","label":"The compound can change the activating signal upstream of an enzyme.","description":"Agmatine induced cytosolic calcium transients in bovine pulmonary artery endothelial cells; repeated exposure downregulated the response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3b6f7c08-a355-540a-a894-685aac98c953","slug":"bovine-pulmonary-endothelial-calcium-transients","display_name":"Bovine pulmonary-endothelial cytosolic calcium transients","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured bovine pulmonary artery endothelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dose was not available in the accessed abstract; no dietary calcium-depletion claim is made.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The compound can change the activating signal upstream of an enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine activation of nitric oxide synthase in endothelial cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9010916/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ca945f72-fb13-5bb6-900a-4429eda79462","evidence_kind":"source_excerpt","locator":"Lines 244-250","start_line":244,"end_line":250,"excerpt":"## agmatine-sulfate-endothelial-calcium\nThe compound can change the activating signal upstream of an enzyme.\nAgmatine induced cytosolic calcium transients in bovine pulmonary artery endothelial cells; repeated exposure downregulated the response.\nModel: Cultured bovine pulmonary artery endothelial cells.\nLimitations: Dose was not available in the accessed abstract; no dietary calcium-depletion claim is made.\nEvidence access: Primary abstract\nAgmatine activation of nitric oxide synthase in endothelial cells. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9010916/","model_system":"Cultured bovine pulmonary artery endothelial cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b55d70e22d07b80964f825770d92fdd1745221420ed8af8d94ca5182b9994509","revision_id":"fc7be029-20e0-5943-b74e-3b06ba694571","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}