{"id":"11035ab4-fca3-5721-89ed-1ca547f236b9","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-paox-acetylspermidine","predicate":"oxidizes","statement":"Recombinant human PAOX oxidized N1-acetylspermidine with Km 2.1 micromolar and kcat 15 per second.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d8bc8dd3-ca1f-5df2-b992-7c0f19a80a2e","mechanism_event_label":"The acetylated molecule has its own enzyme kinetics.","subject":{"id":"80b7a4f6-c2c5-5ee1-8fab-c3500bb5c79c","slug":"paox","display_name":"Human N1-acetylpolyamine oxidase / PAOX","entity_type_key":"protein"},"object":{"id":"69481d06-17fc-50ee-87f6-81c958322f65","slug":"n1-acetylspermidine","display_name":"N1-Acetylspermidine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"d8bc8dd3-ca1f-5df2-b992-7c0f19a80a2e","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-paox-acetylspermidine-event","event_type":"observed_relationship","label":"The acetylated molecule has its own enzyme kinetics.","description":"Recombinant human PAOX oxidized N1-acetylspermidine with Km 2.1 micromolar and kcat 15 per second.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80b7a4f6-c2c5-5ee1-8fab-c3500bb5c79c","slug":"paox","display_name":"Human N1-acetylpolyamine oxidase / PAOX","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"69481d06-17fc-50ee-87f6-81c958322f65","slug":"n1-acetylspermidine","display_name":"N1-Acetylspermidine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not apply these values to unmodified spermidine or a human plasma target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The acetylated molecule has its own enzyme kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Properties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15791459/ · DOI 10.1007/s00280-004-0936-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"59a4c394-b49d-570f-8f7d-62e70fc1778b","evidence_kind":"source_excerpt","locator":"Lines 94-100","start_line":94,"end_line":100,"excerpt":"## spermidine-paox-acetylspermidine\nThe acetylated molecule has its own enzyme kinetics.\nRecombinant human PAOX oxidized N1-acetylspermidine with Km 2.1 micromolar and kcat 15 per second.\nModel: Purified human enzyme.\nLimitations: Do not apply these values to unmodified spermidine or a human plasma target.\nEvidence access: Primary abstract\nProperties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15791459/ · DOI 10.1007/s00280-004-0936-5","model_system":"Purified human enzyme.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}