{"id":"10a778f7-c906-5ef1-bdc7-cefe6d3a23a8","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-catabolic-hydratase","predicate":"processes","statement":"Paenibacillus thiourocanate hydratase had structural features distinguishing it from histidine-pathway urocanate hydratases.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fe4923dd-0cac-5bdc-a12d-848be85daeb9","mechanism_event_label":"A related-looking enzyme handles a different intermediate.","subject":{"id":"ef2cf1f6-957e-5d21-9a31-a04083cd5028","slug":"paenibacillus-thiourocanate-hydratase","display_name":"Paenibacillus sp. thiourocanate hydratase","entity_type_key":"protein"},"object":{"id":"03cbf5fb-f4fe-517e-80fb-f7475c22e441","slug":"thiourocanate","display_name":"Thiourocanate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fe4923dd-0cac-5bdc-a12d-848be85daeb9","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-catabolic-hydratase-event","event_type":"observed_relationship","label":"A related-looking enzyme handles a different intermediate.","description":"Paenibacillus thiourocanate hydratase had structural features distinguishing it from histidine-pathway urocanate hydratases.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ef2cf1f6-957e-5d21-9a31-a04083cd5028","slug":"paenibacillus-thiourocanate-hydratase","display_name":"Paenibacillus sp. thiourocanate hydratase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"03cbf5fb-f4fe-517e-80fb-f7475c22e441","slug":"thiourocanate","display_name":"Thiourocanate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Enzyme structure within the reconstituted bacterial pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not substitute a human histidine-degradation enzyme for this bacterial activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A related-looking enzyme handles a different intermediate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33544568/ · DOI 10.1021/acschembio.0c00968","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1c789bb9-6c25-5c29-86b7-ba6f69ee0adb","evidence_kind":"source_excerpt","locator":"Lines 264-270","start_line":264,"end_line":270,"excerpt":"## ergothioneine-catabolic-hydratase\nA related-looking enzyme handles a different intermediate.\nPaenibacillus thiourocanate hydratase had structural features distinguishing it from histidine-pathway urocanate hydratases.\nModel: Enzyme structure within the reconstituted bacterial pathway.\nLimitations: Do not substitute a human histidine-degradation enzyme for this bacterial activity.\nEvidence access: Primary abstract\nIn Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33544568/ · DOI 10.1021/acschembio.0c00968","model_system":"Enzyme structure within the reconstituted bacterial pathway.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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