{"id":"108b89ff-96ca-5360-b41f-495ac9abce2d","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-mek1","predicate":"inhibits","statement":"Myricetin inhibited MEK1 activity in the mouse JB6 transformation system; GST-MEK1 pull-down supported ATP-noncompetitive binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3dca04ad-f4b3-5298-8f8e-b890e22dacc4","mechanism_event_label":"This kinase experiment differs from ATP-competitive targets.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"85f33887-fcb4-55af-a038-5cf82a88460e","slug":"mouse-map2k1","display_name":"Mouse MEK1 / Map2k1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"3dca04ad-f4b3-5298-8f8e-b890e22dacc4","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-mek1-event","event_type":"observed_relationship","label":"This kinase experiment differs from ATP-competitive targets.","description":"Myricetin inhibited MEK1 activity in the mouse JB6 transformation system; GST-MEK1 pull-down supported ATP-noncompetitive binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"85f33887-fcb4-55af-a038-5cf82a88460e","slug":"mouse-map2k1","display_name":"Mouse MEK1 / Map2k1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse epidermal JB6 P+ cells and GST-MEK1 binding; recombinant construct species not resolved in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cellular node is mouse; do not assume recombinant construct species or selective human inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This kinase experiment differs from ATP-competitive targets.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Myricetin is a novel natural inhibitor of neoplastic cell transformation and MEK1. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17693661/ · DOI 10.1093/carcin/bgm110","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6957f265-2dbe-579a-b85a-397b8c061e49","evidence_kind":"source_excerpt","locator":"Lines 452-458","start_line":452,"end_line":458,"excerpt":"## myricetin-mek1\nThis kinase experiment differs from ATP-competitive targets.\nMyricetin inhibited MEK1 activity in the mouse JB6 transformation system; GST-MEK1 pull-down supported ATP-noncompetitive binding.\nModel: Mouse epidermal JB6 P+ cells and GST-MEK1 binding; recombinant construct species not resolved in accessed abstract.\nLimitations: Cellular node is mouse; do not assume recombinant construct species or selective human inhibition.\nEvidence access: Primary abstract\nMyricetin is a novel natural inhibitor of neoplastic cell transformation and MEK1. · 2007 · https://pubmed.ncbi.nlm.nih.gov/17693661/ · DOI 10.1093/carcin/bgm110","model_system":"Mouse epidermal JB6 P+ cells and GST-MEK1 binding; recombinant construct species not resolved in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}