{"id":"107b7b30-ec8f-58dc-88b5-0380100438c6","stable_key":"a9dd23c6-978a-5755-8bd8-f29bd1fe0cda:nia-clin-arrb1-mouse-flush","predicate":"reduces_response_to_nicotinic_acid","statement":"Arrb1-null mice had less nicotinic-acid-induced flushing while retaining a serum free-fatty-acid response similar to wild-type mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d8fc71ef-7660-5a44-ba69-53b8a4bd8482","mechanism_event_label":"The flushing and fatty-acid responses could be separated in mice; one is not a reliable measure of the other.","subject":{"id":"de855aa8-d1aa-50dc-9587-ff76927481a6","slug":"mouse-arrb1-knockout","display_name":"Mouse Arrb1 knockout genotype","entity_type_key":"gene"},"object":{"id":"b250569e-cba7-5e18-997f-000e56b8a18f","slug":"nicotinic-acid-flushing","display_name":"Nicotinic-acid-induced cutaneous flushing","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d8fc71ef-7660-5a44-ba69-53b8a4bd8482","stable_key":"a9dd23c6-978a-5755-8bd8-f29bd1fe0cda:nia-clin-arrb1-mouse-flush-event","event_type":"biochemical_relationship","label":"The flushing and fatty-acid responses could be separated in mice; one is not a reliable measure of the other.","description":"Arrb1-null mice had less nicotinic-acid-induced flushing while retaining a serum free-fatty-acid response similar to wild-type mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9751fdb6-9b30-586f-b07a-79b8e2cd62dd","slug":"nicotinic-acid","display_name":"Nicotinic acid","entity_type_key":"small_molecule"},"role":"administered_agonist","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"31fe0ea6-e21e-5ad1-bfc6-29e024067c22","slug":"plasma-free-fatty-acid-concentration","display_name":"Plasma free fatty acid concentration","entity_type_key":"cellular_process"},"role":"preserved_separate_endpoint","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"de855aa8-d1aa-50dc-9587-ff76927481a6","slug":"mouse-arrb1-knockout","display_name":"Mouse Arrb1 knockout genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b250569e-cba7-5e18-997f-000e56b8a18f","slug":"nicotinic-acid-flushing","display_name":"Nicotinic-acid-induced cutaneous flushing","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Nicotinic acid (administered_agonist); Plasma free fatty acid concentration (preserved_separate_endpoint)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/niacin-clinical-sources/walters2009.abstract.txt\", \"locator\": \"Indexed primary abstract\", \"file_sha256\": \"2eb822534ed0c8ea9f71ec67288c302e4e375ed9e86cfe70b28f21ae65ffbd4b\", \"start_char\": 0, \"end_char\": 1537, \"text_sha256\": \"2eb822534ed0c8ea9f71ec67288c302e4e375ed9e86cfe70b28f21ae65ffbd4b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell-line receptor signaling and separate Arrb1-null mouse physiology","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Nicotinic-acid stimulation and beta-arrestin perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological receptor signaling, not an essential effect of every B3 precursor. Cell signaling and mouse physiology are distinct arms. Reduced fatty acids or flushing does not establish cardiovascular benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Niacin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"niacin","display_name":"Niacin (vitamin B3)","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The flushing and fatty-acid responses could be separated in mice; one is not a reliable measure of the other.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[nia-clin-walters2009] beta-Arrestin1 mediates nicotinic acid-induced flushing, but not its antilipolytic effect, in mice. 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Cell signaling and mouse physiology are distinct arms. Reduced fatty acids or flushing does not establish cardiovascular benefit.\nexposure: Nicotinic-acid stimulation and beta-arrestin perturbation\ncross_nutrient: Nicotinic acid (administered_agonist); Plasma free fatty acid concentration (preserved_separate_endpoint)\nevidence_span: {\"source_cache\": \"artifacts/niacin-clinical-sources/walters2009.abstract.txt\", \"locator\": \"Indexed primary abstract\", \"file_sha256\": \"2eb822534ed0c8ea9f71ec67288c302e4e375ed9e86cfe70b28f21ae65ffbd4b\", \"start_char\": 0, \"end_char\": 1537, \"text_sha256\": \"2eb822534ed0c8ea9f71ec67288c302e4e375ed9e86cfe70b28f21ae65ffbd4b\"}\n[nia-clin-walters2009] beta-Arrestin1 mediates nicotinic acid-induced flushing, but not its antilipolytic effect, in mice. 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