{"id":"10125b71-d286-5fe5-8007-0d088630b34b","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-ckb-mitochondrial-trafficking","predicate":"supports","statement":"CKB trafficked to adipocyte mitochondria through an internal mitochondrial targeting sequence and supported the futile creatine cycle.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0b8b8222-9520-56b7-b3d5-8ff83f3157bb","mechanism_event_label":"In fat cells, CKB can move to mitochondria and help run an energy-consuming creatine cycle.","subject":{"id":"e86c3ea5-b8e5-5e65-829e-41185cc4ca36","slug":"mouse-ckb","display_name":"Mouse creatine kinase B / Ckb","entity_type_key":"protein"},"object":{"id":"6b11ed38-75d0-5723-87d0-8419dc74f3d6","slug":"adipocyte-creatine-cycle","display_name":"Adipocyte futile creatine cycle","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0b8b8222-9520-56b7-b3d5-8ff83f3157bb","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-ckb-mitochondrial-trafficking-event","event_type":"biochemical_relationship","label":"In fat cells, CKB can move to mitochondria and help run an energy-consuming creatine cycle.","description":"CKB trafficked to adipocyte mitochondria through an internal mitochondrial targeting sequence and supported the futile creatine cycle.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c081984a-e118-582f-ac46-6a767f8f0bf2","slug":"phosphocreatine","display_name":"Phosphocreatine","entity_type_key":"small_molecule"},"role":"intermediate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e86c3ea5-b8e5-5e65-829e-41185cc4ca36","slug":"mouse-ckb","display_name":"Mouse creatine kinase B / Ckb","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b11ed38-75d0-5723-87d0-8419dc74f3d6","slug":"adipocyte-creatine-cycle","display_name":"Adipocyte futile creatine cycle","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/creatine-research/33597756.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"90b9ec2286a126569876b65a77450dec9f600bf04cb6f3e8c4aeb43edcc34c4b\", \"start_char\": 0, \"end_char\": 942, \"text_sha256\": \"90b9ec2286a126569876b65a77450dec9f600bf04cb6f3e8c4aeb43edcc34c4b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adipocyte biochemical experiments and conditional gene deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Thermogenic stimulation and adipocyte Ckb loss","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human cell induction and mouse causal phenotypes are separately scoped; no human weight-loss intervention was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Creatine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse adipocyte mechanistic experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In fat cells, CKB can move to mitochondria and help run an energy-consuming creatine cycle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[creatine-p33597756] Creatine kinase B controls futile creatine cycling in thermogenic fat. (2021). https://pubmed.ncbi.nlm.nih.gov/33597756/ DOI: 10.1038/s41586-021-03221-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Thermogenic adipose tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"595bd819-d169-57ac-bf55-c686f010bf77","evidence_kind":"source_excerpt","locator":"Lines 477-488","start_line":477,"end_line":488,"excerpt":"### creatine-ckb-mitochondrial-trafficking\nCKB trafficked to adipocyte mitochondria through an internal mitochondrial targeting sequence and supported the futile creatine cycle.\nCondition category: normal\nnutrient_topic: Creatine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In fat cells, CKB can move to mitochondria and help run an energy-consuming creatine cycle.\norganism: Mouse adipocyte mechanistic experiments\ntissue_or_cell_type: Thermogenic adipose tissue\nexperimental_model: Adipocyte biochemical experiments and conditional gene deletion\nlimitations: Human cell induction and mouse causal phenotypes are separately scoped; no human weight-loss intervention was tested.\nexposure: Thermogenic stimulation and adipocyte Ckb loss\nevidence_span: {\"source_cache\": \"artifacts/creatine-research/33597756.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"90b9ec2286a126569876b65a77450dec9f600bf04cb6f3e8c4aeb43edcc34c4b\", \"start_char\": 0, \"end_char\": 942, \"text_sha256\": \"90b9ec2286a126569876b65a77450dec9f600bf04cb6f3e8c4aeb43edcc34c4b\"}\n[creatine-p33597756] Creatine kinase B controls futile creatine cycling in thermogenic fat. 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