{"id":"0ff90024-4fc8-5253-af75-3ae33038b020","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-hoxb1-ra-enhancer","predicate":"regulates","statement":"A conserved Hoxb1 enhancer containing a RARE mediated RA responsiveness and early neuroectodermal expression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"9938dda5-8584-5988-a181-f28e1cf210be","mechanism_event_label":"Embryonic RA signaling connects to a spatial developmental gene program.","subject":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"aa8c2c90-0447-5c92-aa6d-a32eb78f6b0c","slug":"hoxb1-early-neuroectoderm-expression","display_name":"Early neuroectodermal Hoxb1 expression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9938dda5-8584-5988-a181-f28e1cf210be","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-hoxb1-ra-enhancer-event","event_type":"biochemical_relationship","label":"Embryonic RA signaling connects to a spatial developmental gene program.","description":"A conserved Hoxb1 enhancer containing a RARE mediated RA responsiveness and early neuroectodermal expression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ce392c9e-6483-5914-b18b-d6a2520dbbf6","slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"},"role":"nutrient-context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"97eaeb16-8d39-5ac3-a5a8-75de142c70a1","slug":"hoxb1-gene","display_name":"Hoxb1 gene","entity_type_key":"gene"},"role":"target-locus","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"aa8c2c90-0447-5c92-aa6d-a32eb78f6b0c","slug":"hoxb1-early-neuroectoderm-expression","display_name":"Early neuroectodermal Hoxb1 expression","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_locator","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse Hoxb1 enhancer transgenes; homologous chicken and pufferfish elements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enhancer transgenes and RA exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Enhancer behavior is not a maternal serum-retinol threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus; comparative chicken/pufferfish enhancer sequences","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Embryonic RA signaling connects to a spatial developmental gene program.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-marshall-1994] A conserved retinoic acid response element required for early expression of the homeobox gene Hoxb-1 (1994). https://www.nature.com/articles/370567a0 DOI: 10.1038/370567a0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Early embryonic neuroectoderm","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"42eb4798-db2f-5dce-abe4-cb06bf6d708c","evidence_kind":"source_excerpt","locator":"Lines 1396-1407","start_line":1396,"end_line":1407,"excerpt":"### va-sig-hoxb1-ra-enhancer\nA conserved Hoxb1 enhancer containing a RARE mediated RA responsiveness and early neuroectodermal expression.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Embryonic RA signaling connects to a spatial developmental gene program.\norganism: Mus musculus; comparative chicken/pufferfish enhancer sequences\ntissue_or_cell_type: Early embryonic neuroectoderm\nexperimental_model: Mouse Hoxb1 enhancer transgenes; homologous chicken and pufferfish elements.\nlimitations: Enhancer behavior is not a maternal serum-retinol threshold.\nevidence_locator: Abstract\nexposure: Enhancer transgenes and RA exposure\n[va-marshall-1994] A conserved retinoic acid response element required for early expression of the homeobox gene Hoxb-1 (1994). https://www.nature.com/articles/370567a0 DOI: 10.1038/370567a0","model_system":"Mouse Hoxb1 enhancer transgenes; homologous chicken and pufferfish elements.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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