{"id":"0fef8b5c-b0e0-5768-afa8-28664431a29f","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-gsh-icam-rescue","predicate":"attenuates_tested_suppression_of","statement":"Adding glutathione attenuated cucurbitacin B inhibition of TNF-alpha-induced ICAM1 expression; L-cysteine had a smaller effect than the other tested thiols.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"035036a8-599d-5a7d-8a67-3a320c12bc0e","mechanism_event_label":"A separately stored thiol changed the signaling result.","subject":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"object":{"id":"73fdab07-975f-5669-8cf4-298634556a04","slug":"human-icam1","display_name":"Human intercellular adhesion molecule 1 / ICAM1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"035036a8-599d-5a7d-8a67-3a320c12bc0e","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-gsh-icam-rescue-event","event_type":"observed_relationship","label":"A separately stored thiol changed the signaling result.","description":"Adding glutathione attenuated cucurbitacin B inhibition of TNF-alpha-induced ICAM1 expression; 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it does not establish a human supplement interaction magnitude.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A separately stored thiol changed the signaling result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin B Down-Regulates TNF Receptor 1 Expression and Inhibits the TNF-α-Dependent Nuclear Factor κB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35806134/ · DOI 10.3390/ijms23137130","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fe7f2647-15b9-5cbd-8f84-2480f7f380d4","evidence_kind":"source_excerpt","locator":"Lines 220-226","start_line":220,"end_line":226,"excerpt":"## cucurbitacin-b-gsh-icam-rescue\nA separately stored thiol changed the signaling result.\nAdding glutathione attenuated cucurbitacin B inhibition of TNF-alpha-induced ICAM1 expression; L-cysteine had a smaller effect than the other tested thiols.\nModel: Human A549 cytokine-stimulation experiment.\nLimitations: The experiment supports a chemical-reactivity dependency; it does not establish a human supplement interaction magnitude.\nEvidence access: Primary abstract\nCucurbitacin B Down-Regulates TNF Receptor 1 Expression and Inhibits the TNF-α-Dependent Nuclear Factor κB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35806134/ · DOI 10.3390/ijms23137130","model_system":"Human A549 cytokine-stimulation experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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