{"id":"0fe42e3b-3d48-58c1-ae18-0a806303f88c","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-mouse-metastatic-catabolism","predicate":"supports","statement":"Pharmacological inhibition of Prodh impaired lung-metastasis formation in orthotopic 4T1 and EMT6.5 mouse breast-cancer models.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"702531de-c8dc-568b-8a5e-695065c13892","mechanism_event_label":"A proline-consuming pathway can support growth in a metastatic setting.","subject":{"id":"3c8f99f8-4ce4-5355-ac90-9c371bf0257e","slug":"mouse-prodh","display_name":"Mouse proline dehydrogenase / Prodh","entity_type_key":"protein"},"object":{"id":"ac5120bf-a775-50f0-b5f2-1aa8c19b4b1b","slug":"mouse-prodh-lung-metastasis","display_name":"Lung-metastasis formation in mouse breast-cancer models","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"702531de-c8dc-568b-8a5e-695065c13892","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-mouse-metastatic-catabolism-event","event_type":"observed_relationship","label":"A proline-consuming pathway can support growth in a metastatic setting.","description":"Pharmacological inhibition of Prodh impaired lung-metastasis formation in orthotopic 4T1 and EMT6.5 mouse breast-cancer models.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3c8f99f8-4ce4-5355-ac90-9c371bf0257e","slug":"mouse-prodh","display_name":"Mouse proline dehydrogenase / Prodh","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ac5120bf-a775-50f0-b5f2-1aa8c19b4b1b","slug":"mouse-prodh-lung-metastasis","display_name":"Lung-metastasis formation in mouse breast-cancer models","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse breast-cancer models, complemented by three-dimensional culture and human tumor-expression comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not establish that dietary proline causes metastasis or that proline restriction is a proven treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A proline-consuming pathway can support growth in a metastatic setting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Proline metabolism supports metastasis formation and could be inhibited to selectively target metastasizing cancer cells. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28492237/ · DOI 10.1038/ncomms15267","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"edb25f40-4e88-5aee-9928-2b854d5bb751","evidence_kind":"source_excerpt","locator":"Lines 382-388","start_line":382,"end_line":388,"excerpt":"## l-proline-mouse-metastatic-catabolism\nA proline-consuming pathway can support growth in a metastatic setting.\nPharmacological inhibition of Prodh impaired lung-metastasis formation in orthotopic 4T1 and EMT6.5 mouse breast-cancer models.\nModel: Mouse breast-cancer models, complemented by three-dimensional culture and human tumor-expression comparisons.\nLimitations: This does not establish that dietary proline causes metastasis or that proline restriction is a proven treatment.\nEvidence access: Primary abstract\nProline metabolism supports metastasis formation and could be inhibited to selectively target metastasizing cancer cells. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28492237/ · DOI 10.1038/ncomms15267","model_system":"Mouse breast-cancer models, complemented by three-dimensional culture and human tumor-expression comparisons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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