{"id":"0f9c2e28-3629-5517-aa98-806bade8b934","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-rdh10-rod-transgene-9cis-retinol","predicate":"does_not_enable_measured","statement":"Ectopic RDH10 expression did not enable bleached mouse rods to recover sensitivity when supplied with 9-cis-retinol.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c3d7fe03-feaf-5d8c-9e8e-839c4549f905","mechanism_event_label":"Adding RDH10 alone did not let rods use the supplied retinol isomer.","subject":{"id":"965f656c-945c-582d-99c4-fc89f14bac9a","slug":"rdh10-rod-transgenic","display_name":"Rod-expressed transgenic RDH10","entity_type_key":"protein_state"},"object":{"id":"cc277d25-a552-5c47-9c82-a642fbc2c4a8","slug":"rod-dark-adaptation","display_name":"Rod dark adaptation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c3d7fe03-feaf-5d8c-9e8e-839c4549f905","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-rdh10-rod-transgene-9cis-retinol-event","event_type":"observed_intervention","label":"Adding RDH10 alone did not let rods use the supplied retinol isomer.","description":"Ectopic RDH10 expression did not enable bleached mouse rods to recover sensitivity when supplied with 9-cis-retinol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"965f656c-945c-582d-99c4-fc89f14bac9a","slug":"rdh10-rod-transgenic","display_name":"Rod-expressed transgenic RDH10","entity_type_key":"protein_state"},"role":"ectopic protein state","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89b4b04a-7637-56f4-ac55-501ad31d1f61","slug":"rdh10","display_name":"Retinol dehydrogenase 10 / RDH10","entity_type_key":"protein"},"role":"expressed protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"da6a9b49-7a18-5c27-b7a2-7c6edae2e49f","slug":"9-cis-retinol","display_name":"9-cis-retinol","entity_type_key":"small_molecule"},"role":"supplied substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"cc277d25-a552-5c47-9c82-a642fbc2c4a8","slug":"rod-dark-adaptation","display_name":"Rod dark adaptation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 9C; Results: ectopic RDH10 expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rod RDH10 transgenesis; single-cell suction recordings","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 micromolar 9-cis-retinol after approximately 50% pigment bleach.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression was mosaic; the substrate was 9-cis-retinol, not 11-cis-retinol. This tests sufficiency in rods, not necessity in cones.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin A","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding RDH10 alone did not let rods use the supplied retinol isomer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vav-xue2017] The role of retinol dehydrogenase 10 in the cone visual cycle. (2017). https://pubmed.ncbi.nlm.nih.gov/28539612/ DOI: 10.1038/s41598-017-02549-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"isolated rods","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a121a73a-5e64-5324-a5e9-f8f51442accc","evidence_kind":"source_excerpt","locator":"Lines 663-676","start_line":663,"end_line":676,"excerpt":"### vae-rdh10-rod-transgene-9cis-retinol\nEctopic RDH10 expression did not enable bleached mouse rods to recover sensitivity when supplied with 9-cis-retinol.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Adding RDH10 alone did not let rods use the supplied retinol isomer.\norganism: Mus musculus\ntissue_or_cell_type: isolated rods\nexperimental_model: Rod RDH10 transgenesis; single-cell suction recordings\nlimitations: Expression was mosaic; the substrate was 9-cis-retinol, not 11-cis-retinol. This tests sufficiency in rods, not necessity in cones.\nexposure: 100 micromolar 9-cis-retinol after approximately 50% pigment bleach.\ncross_nutrient: false\nevidence_location: Figure 9C; Results: ectopic RDH10 expression\nnutrient: Vitamin A\n[vav-xue2017] The role of retinol dehydrogenase 10 in the cone visual cycle. (2017). https://pubmed.ncbi.nlm.nih.gov/28539612/ DOI: 10.1038/s41598-017-02549-8","model_system":"Rod RDH10 transgenesis; single-cell suction recordings","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vav-xue2017] The role of retinol dehydrogenase 10 in the cone visual cycle. 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