{"id":"0f8e7bc6-4415-58d1-95e2-6a7a64a53bbb","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ctns-ragulator","predicate":"engages_conformation_dependently","statement":"The human CTNS N288K variant altered conformation and suppressed engagement with Ragulator–Rag, linking transporter state with a nutrient-signaling complex.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2c2c0bfc-49ee-598d-af6a-7908b8100812","mechanism_event_label":"Transport machinery can also affect how the cell senses nutrient availability.","subject":{"id":"80987fa1-4f0c-5456-a6e2-d7f7d2d493b3","slug":"ctns","display_name":"Human lysosomal cystine transporter / CTNS","entity_type_key":"protein"},"object":{"id":"9b626188-8347-59f1-9776-c1f1cbf5f67a","slug":"human-ragulator-rag-complex","display_name":"Human Ragulator–Rag complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"2c2c0bfc-49ee-598d-af6a-7908b8100812","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-ctns-ragulator-event","event_type":"observed_relationship","label":"Transport machinery can also affect how the cell senses nutrient availability.","description":"The human CTNS N288K variant altered conformation and suppressed engagement with Ragulator–Rag, linking transporter state with a nutrient-signaling complex.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80987fa1-4f0c-5456-a6e2-d7f7d2d493b3","slug":"ctns","display_name":"Human lysosomal cystine transporter / CTNS","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9b626188-8347-59f1-9776-c1f1cbf5f67a","slug":"human-ragulator-rag-complex","display_name":"Human Ragulator–Rag complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cystinosin cryo-EM and interaction experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not demonstrate that oral cysteine directly activates mTORC1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Transport machinery can also affect how the cell senses nutrient availability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structure and mechanism of human cystine exporter cystinosin. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36113465/ · DOI 10.1016/j.cell.2022.08.020","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"81214929-f33f-5fd5-8092-0f0d6c6ef87a","evidence_kind":"source_excerpt","locator":"Lines 52-58","start_line":52,"end_line":58,"excerpt":"## l-cysteine-ctns-ragulator\nTransport machinery can also affect how the cell senses nutrient availability.\nThe human CTNS N288K variant altered conformation and suppressed engagement with Ragulator–Rag, linking transporter state with a nutrient-signaling complex.\nModel: Human cystinosin cryo-EM and interaction experiments.\nLimitations: This does not demonstrate that oral cysteine directly activates mTORC1.\nEvidence access: Primary full text\nStructure and mechanism of human cystine exporter cystinosin. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36113465/ · DOI 10.1016/j.cell.2022.08.020","model_system":"Human cystinosin cryo-EM and interaction experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f722669e54eab08ffe7289f9d79ddfc443014ed8c4bc5ba10ce2635470d22498","revision_id":"8ae25609-a031-5782-9c62-6cad8767ea46","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}