{"id":"0f77896a-5d80-5851-bfb2-9792d7fe04ba","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-arh-targets-romk-endocytosis","predicate":"promotes","statement":"ARH bound the ROMK internalization motif and promoted endocytosis in COS-7 cells; Arh deletion altered dietary regulation of renal ROMK.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6bd16d6c-c48e-5842-af9f-01939885a643","mechanism_event_label":"An adaptor helps pull ROMK from the membrane and contributes to potassium-dependent channel adjustment.","subject":{"id":"13b7ac9d-5aac-53eb-a472-062eab308bb2","slug":"ldlrap1","display_name":"Low-density lipoprotein receptor adaptor protein 1","entity_type_key":"protein"},"object":{"id":"22c568e4-bb53-5d51-96b5-8ec2a46a5bf7","slug":"romk-endocytosis","display_name":"ROMK endocytosis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6bd16d6c-c48e-5842-af9f-01939885a643","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-arh-targets-romk-endocytosis-event","event_type":"biochemical_relationship","label":"An adaptor helps pull ROMK from the membrane and contributes to potassium-dependent channel adjustment.","description":"ARH bound the ROMK internalization motif and promoted endocytosis in COS-7 cells; 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Arh deletion altered dietary regulation of renal ROMK.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An adaptor helps pull ROMK from the membrane and contributes to potassium-dependent channel adjustment.\norganism: Chlorocebus-derived COS-7 cells; Mus musculus\ntissue_or_cell_type: Cell system and distal nephron\nexperimental_model: Binding, knockdown and Arh-null dietary experiments\nlimitations: Cell uptake mechanism and mouse dietary phenotype are complementary experiments.\nevidence_location: Abstract; binding motif experiments and renal Arh-null comparison.\n[fang-2009-arh-romk] The ARH adaptor protein regulates endocytosis of the ROMK potassium secretory channel in mouse kidney (2009). https://pmc.ncbi.nlm.nih.gov/articles/PMC2769171/ DOI: 10.1172/JCI37950","model_system":"Binding, knockdown and Arh-null dietary experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fang-2009-arh-romk] The ARH adaptor protein regulates endocytosis of the ROMK potassium secretory channel in mouse kidney (2009). https://pmc.ncbi.nlm.nih.gov/articles/PMC2769171/ DOI: 10.1172/JCI37950","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}