{"id":"0f61719f-01fa-5376-ba85-a563985d741c","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:ascorbate-lysyl-hydroxylase","predicate":"supports_sustained_activity_of","statement":"Ascorbate supports sustained lysyl-hydroxylase activity; the enzyme can initially turn over without ascorbate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0fcc82ca-53a5-5e8d-a1c1-874ad112a3e3","mechanism_event_label":"Vitamin C supports the reaction, but is not consumed in every coupled turnover.","subject":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"object":{"id":"a11a1a13-9184-57b3-b5bf-45708f4c3227","slug":"lysyl-hydroxylases","display_name":"Collagen lysyl hydroxylase family","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"0fcc82ca-53a5-5e8d-a1c1-874ad112a3e3","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:ascorbate-lysyl-hydroxylase-event","event_type":"biochemical_relationship","label":"Vitamin C supports the reaction, but is not consumed in every coupled turnover.","description":"Ascorbate supports sustained lysyl-hydroxylase activity; the enzyme can initially turn over without ascorbate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a11a1a13-9184-57b3-b5bf-45708f4c3227","slug":"lysyl-hydroxylases","display_name":"Collagen lysyl hydroxylase family","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"34b151b4-591b-5008-bc84-5f1d610d102b","slug":"collagen-lysine-residues","display_name":"Collagen-bound lysine residues","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"cosubstrate","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified chick-embryo enzyme kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Family-level enzyme preparation, not a human PLOD isoform comparison or lysine-supplement trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Chicken","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin C supports the reaction, but is not consumed in every coupled turnover.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[plod-cofactor-1980] Studies on the lysyl hydroxylase reaction. I. Initial velocity kinetics and related aspects. (1980). https://pubmed.ncbi.nlm.nih.gov/6766066/ DOI: 10.1016/0005-2744(80)90040-6","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Not specified as a whole tissue; see experimental model.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dd24fbb0-d09c-595d-b56d-f4c2a59de858","evidence_kind":"source_excerpt","locator":"Lines 427-435","start_line":427,"end_line":435,"excerpt":"### ascorbate-lysyl-hydroxylase\nAscorbate supports sustained lysyl-hydroxylase activity; the enzyme can initially turn over without ascorbate.\nPlain language: Vitamin C supports the reaction, but is not consumed in every coupled turnover.\nCondition category: normal\norganism: Chicken\ntissue_or_cell_type: Not specified as a whole tissue; see experimental model.\nexperimental_model: Purified chick-embryo enzyme kinetics.\nlimitations: Family-level enzyme preparation, not a human PLOD isoform comparison or lysine-supplement trial.\n[plod-cofactor-1980] Studies on the lysyl hydroxylase reaction. 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