{"id":"0f4303b4-dcc5-5bff-9d3d-8e83b8950fa8","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-copper-fenton","predicate":"reduces","statement":"Luteolin–copper coordination suppressed radical formation and luteolin dose-dependently protected DNA in the Cu-Fenton assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fc30039c-16fd-5ba9-866a-d51d1b83478d","mechanism_event_label":"This chemical assay showed protection rather than inevitable metal-driven damage.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"5a61afec-5eca-57a1-bffa-5d3cdf9935ae","slug":"copper-fenton-dna-damage","display_name":"Cu-Fenton-system radical formation and DNA damage","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fc30039c-16fd-5ba9-866a-d51d1b83478d","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-copper-fenton-event","event_type":"observed_relationship","label":"This chemical assay showed protection rather than inevitable metal-driven damage.","description":"Luteolin–copper coordination suppressed radical formation and luteolin dose-dependently protected DNA in the Cu-Fenton assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5a61afec-5eca-57a1-bffa-5d3cdf9935ae","slug":"copper-fenton-dna-damage","display_name":"Cu-Fenton-system radical formation and DNA damage","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"EPR spin trapping and DNA gel electrophoresis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Neither cancer prevention nor clinical copper depletion follows from this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This chemical assay showed protection rather than inevitable metal-driven damage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The effect of Luteolin on DNA damage mediated by a copper catalyzed Fenton reaction. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34717250/ · DOI 10.1016/j.jinorgbio.2021.111635","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"239efc4d-8419-5963-9c0b-4d71d3f7853b","evidence_kind":"source_excerpt","locator":"Lines 324-330","start_line":324,"end_line":330,"excerpt":"## luteolin-copper-fenton\nThis chemical assay showed protection rather than inevitable metal-driven damage.\nLuteolin–copper coordination suppressed radical formation and luteolin dose-dependently protected DNA in the Cu-Fenton assay.\nModel: EPR spin trapping and DNA gel electrophoresis.\nLimitations: Neither cancer prevention nor clinical copper depletion follows from this assay.\nEvidence access: Primary abstract\nThe effect of Luteolin on DNA damage mediated by a copper catalyzed Fenton reaction. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34717250/ · DOI 10.1016/j.jinorgbio.2021.111635","model_system":"EPR spin trapping and DNA gel electrophoresis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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