{"id":"0f26132a-7850-517c-b8fc-d88edd88619f","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-sqle","predicate":"inhibits_tested","statement":"SAC inhibited purified recombinant human squalene monooxygenase with an IC50 of 110 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ea0623a9-ff59-53cd-97a8-ebaf3c072064","mechanism_event_label":"A downstream cholesterol enzyme was tested directly.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"923a6f4a-e004-5fa5-a434-5e7f78c543c2","slug":"sqle","display_name":"Human squalene monooxygenase / SQLE","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ea0623a9-ff59-53cd-97a8-ebaf3c072064","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-sqle-event","event_type":"observed_relationship","label":"A downstream cholesterol enzyme was tested directly.","description":"SAC inhibited purified recombinant human squalene monooxygenase with an IC50 of 110 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"923a6f4a-e004-5fa5-a434-5e7f78c543c2","slug":"sqle","display_name":"Human squalene monooxygenase / SQLE","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"SAC IC50 110 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Time-dependent inhibition; duration not retrieved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC and other compounds tested individually; thiol reversal experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant squalene monooxygenase assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Enzyme-assay potency does not establish inhibition at oral human exposures; not interchangeable with HMGCR inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human recombinant protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A downstream cholesterol enzyme was tested directly.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[11385050] Garlic and garlic-derived compounds inhibit human squalene monooxygenase. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11385050/ · DOI 10.1093/jn/131.6.1662","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell-free enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant squalene monooxygenase assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"46b18ab4-220d-5a6f-9b63-158e59acaa49","evidence_kind":"source_excerpt","locator":"Lines 221-228","start_line":221,"end_line":228,"excerpt":"## s-allylcysteine-sqle\nA downstream cholesterol enzyme was tested directly.\nSAC inhibited purified recombinant human squalene monooxygenase with an IC50 of 110 micromolar.\nModel: Purified recombinant squalene monooxygenase assay\nLimitations: Enzyme-assay potency does not establish inhibition at oral human exposures; not interchangeable with HMGCR inhibition.\nEvidence access: Primary abstract\n[11385050] Garlic and garlic-derived compounds inhibit human squalene monooxygenase. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11385050/ · DOI 10.1093/jn/131.6.1662\nStructured context: {\"organism\": \"Human recombinant protein\", \"tissue_or_cell_type\": \"Purified recombinant squalene monooxygenase assay\", \"dose\": \"SAC IC50 110 micromolar\", \"duration\": \"Time-dependent inhibition; duration not retrieved\", \"route\": \"Cell-free enzyme assay\", \"experimental_comparison\": \"SAC and other compounds tested individually; thiol reversal experiments\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Purified recombinant squalene monooxygenase assay","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"91d7aeac82e42ce559d13dd8a2e0165d37760f7f555dd01c80e0604fa6e71ea2","revision_id":"545e497c-e5e3-543c-b26b-b297cbc7bbec","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}