{"id":"0f226608-3cc7-56f9-b89b-e46629882c8b","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-islet-leucine-combination","predicate":"augments_nutrient_evoked","statement":"Alanine augmented insulin release evoked by leucine or 2-ketoisocaproate in normal adult rat islets, while not significantly changing glucose-induced secretion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9b6e586c-f750-5098-baae-d70d4d79f994","mechanism_event_label":"The accompanying nutrient changed whether alanine increased insulin.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"1a83f9c7-c2cb-533d-a25b-e51fbeee032f","slug":"rat-normal-islet-insulin","display_name":"Insulin secretion from normal adult rat islets","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9b6e586c-f750-5098-baae-d70d4d79f994","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-islet-leucine-combination-event","event_type":"observed_relationship","label":"The accompanying nutrient changed whether alanine increased insulin.","description":"Alanine augmented insulin release evoked by leucine or 2-ketoisocaproate in normal adult rat islets, while not significantly changing glucose-induced secretion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1a83f9c7-c2cb-533d-a25b-e51fbeee032f","slug":"rat-normal-islet-insulin","display_name":"Insulin secretion from normal adult rat islets","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bbdfd11c-42dd-520e-8a1f-7fe3d19e3e0e","slug":"alpha-ketoisocaproate","display_name":"4-Methyl-2-oxopentanoate / alpha-ketoisocaproate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated normal adult rat islets.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This setting differs from clonal cells; model and substrate conditions are retained rather than treated as an unexplained contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The accompanying nutrient changed whether alanine increased insulin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The stimulus-secretion coupling of amino acid-induced insulin release. Insulinotropic action of L-alanine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12383948/ · DOI 10.1016/s0304-4165(02)00337-9","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"93266826-f19d-53d7-b7fe-6580f733c935","evidence_kind":"source_excerpt","locator":"Lines 376-382","start_line":376,"end_line":382,"excerpt":"## alanine-islet-leucine-combination\nThe accompanying nutrient changed whether alanine increased insulin.\nAlanine augmented insulin release evoked by leucine or 2-ketoisocaproate in normal adult rat islets, while not significantly changing glucose-induced secretion.\nModel: Isolated normal adult rat islets.\nLimitations: This setting differs from clonal cells; model and substrate conditions are retained rather than treated as an unexplained contradiction.\nEvidence access: Primary abstract\nThe stimulus-secretion coupling of amino acid-induced insulin release. Insulinotropic action of L-alanine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12383948/ · DOI 10.1016/s0304-4165(02)00337-9","model_system":"Isolated normal adult rat islets.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}